Expression of the Clonostachys rosea lactonohydrolase gene by Lactobacillus reuteri to increase its zearalenone-removing ability

• Published in: Microbial cell factories 2017-04, Vol.16 (1), p.69-69, Article 69
• Main Authors: Yang, Wen-Chun, Hsu, Tsui-Chun, Cheng, Kuan-Chen, Liu, Je-Ruei
• Format: Article
• Language: English
• Subjects: Additives; Adhesion; Aflatoxin B1; Agricultural commodities; Agricultural practices; Agriculture; Agronomy; Anchoring; Animals; Antibodies; Antigens; Ascomycota; Assembly; Bacteria; Bacterial Adhesion; Bile salts; Cellulase; Chickens - microbiology; Cloning; Cloning, Molecular; Contamination; Cytokines; Decontamination; Deoxyribonucleic acid; DNA; E coli; Enzymes; Escherichia coli - genetics; Exposure; Fermentation; Food contamination & poisoning; Fragmentation; Fungi; Gastrointestinal tract; Gastrointestinal Tract - microbiology; Gene Expression; Genetic aspects; Genetic Vectors; Hydrolases; Hydrolases - genetics; Hydrolases - metabolism; Hypocreales - enzymology; Hypocreales - genetics; Immunoglobulin G; Immunoglobulins; Intestine; Laboratories; Lactobacillus reuteri; Lactobacillus reuteri - enzymology; Lactobacillus reuteri - genetics; Lactobacillus reuteri - growth & development; Lactobacillus reuteri - metabolism; Lactones; Lactonohydrolase; Lymphoid tissue; Metabolism; Metabolites; Microflora; Mycotoxins; Nutritive value; Organs; Plasmids; Probiotics; Proteins; Quality; Ribonucleic acid; RNA; Rumen; Salt tolerance; Secondary metabolites; Sourdough; Structural proteins; Zearalenone; Zearalenone - metabolism; Zeranol
• ISSN: 1475-2859; 1475-2859
• DOI: 10.1186/s12934-017-0687-8

Mycotoxins are secondary metabolites produced by filamentous fungi that can contaminate agricultural crops in the field as well as during harvest, transportation, processing, or storage. Zearalenone (ZEN), a non-steroidal estrogenic mycotoxin, produced by Fusarium species, has been shown to be associated with reproductive disorders in farm animals and to a lesser extent in hyperoestrogenic syndromes in humans. Thus, the decontamination of ZEN in foods and feeds is an important issue. In this study, the gene encoding ZHD101, a ZEN-degrading enzyme produced by Clonostachys rosea IFO 7063, was cloned into an Escherichia coli-Lactobacillus shuttle vector, pNZ3004, and the resultant plasmid pNZ-zhd101 was then introduced via electroporation into Lactobacillus reuteri Pg4, a probiotic strain isolated from the gastrointestinal tract of broilers. The transformed strain L. reuteri pNZ-zhd101 acquired the capacity to degrade ZEN. In addition, the production of recombinant ZHD101 did not affect cell growth, acid and bile salt tolerance, and had only a minor effect on the adhesion ability of L. reuteri pNZ-zhd101. To the best of our knowledge, this is the first report of successful expression of a ZEN-degrading enzyme by intestinal lactobacilli.