Dietary Thymol Supplementation Promotes Antioxidant Responses and Thermal Stress Resistance in Rainbow Trout, Oncorhynchus mykiss

• Published in: Animals (Basel) 2024-10, Vol.14 (20), p.2988
• Main Authors: Yousefi, Morteza, Hoseini, Seyyed Morteza, Vatnikov, Yury Anatolyevich, Karamyan, Arfenya, Kulikov, Evgeny Vladimirovich
• Format: Article
• Language: English
• Subjects: Antioxidants; Aquaculture; Aquariums; Biomass; Cold; Diet; Efficiency; Fish; functional feed; Glucose; Heat; Hormones; immunology; nano-liposome; Nitrogen; nutrition; Plasma; Proteins; Tilapia; Trout; Water temperature
• ISSN: 2076-2615; 2076-2615
• DOI: 10.3390/ani14202988

Rainbow trout fingerlings were fed, in triplicate, diets supplemented with 0 (CTL), 50 (50 TM), 100 (100 TM), 200 (200 TM), 400 (400 TM) and 800 (800 TM) mg/kg of thymol, followed by 48 h of thermal stress. Growth performance and humoral immunological parameters showed no significant responses to dietary thymol concentrations. Fish fed 50-400 mg/kg thymol diets had significantly higher survival after heat stress. Plasma cortisol, glucose, hepatic glutathione peroxidase, glutathione reductase and erythrocyte catalase significantly increased after thermal stress, whereas total plasma antioxidant capacity, ascorbate, and hepatic/erythrocyte reduced-glutathione significantly decreased. There were significant elevations in plasma ascorbate and hepatic glutathione reductase in the 50 TM, 100 TM and 200 TM groups; plasma total antioxidant capacity in the 100 TM and 200 TM groups; hepatic glutathione peroxidase in the 200 TM group; and hepatic-reduced glutathione in the 100 TM, 200 TM and 400 TM groups, compared to CTL. The highest hepatic superoxide dismutase and lowest hepatic malondialdehyde were observed in the 100 TM group before heat stress. These parameters significantly increased after thermal stress in the treatment groups, except in the 100 TM and 200 TM groups. Hepatic catalase showed no significant difference among the treatment groups before thermal stress. Hepatic catalase significantly increased after heat stress in all treatment groups, except in the 100 TM group. Erythrocyte superoxide dismutase significantly increased in the 100 TM group before heat stress, whereas erythrocyte malondialdehyde significantly decreased in the 100 TM and 200 TM groups after thermal stress. Based on the results, 100 mg/kg of thymol can promote antioxidant power and thermal stress resistance in rainbow trout.