Assessment of Ceragenins in Prevention of Damage to Voice Prostheses Caused by Candida Biofilm Formation

This study aimed to investigate the potential application of ceragenins (CSAs) as new candidacidal agents to prevent biofilm formation on voice prostheses (VPs). The deterioration of the silicone material of VPs is caused by biofilm growth on the device which leads to frequent replacement procedures...

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Bibliographic Details
Published in:Pathogens (Basel) 2021-10, Vol.10 (11), p.1371
Main Authors: Spałek, Jakub, Daniluk, Tamara, Godlewski, Adrian, Deptuła, Piotr, Wnorowska, Urszula, Ziembicka, Dominika, Cieśluk, Mateusz, Fiedoruk, Krzysztof, Ciborowski, Michał, Krętowski, Adam, Góźdź, Stanisław, Durnaś, Bonita, Savage, Paul B., Okła, Sławomir, Bucki, Robert
Format: Article
Language:English
Subjects:
Alcohol
Antimicrobial agents
Bacteria
biofilm
Biofilms
Candida
Candida spp
Catheters
ceragenins
Damage prevention
Drug resistance
Esophagus
Ethanol
Fistula
Fungi
Gram-positive bacteria
impregnation
In vitro methods and tests
In vivo methods and tests
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Medical equipment
Minimum inhibitory concentration
Nosocomial infections
Prostheses
Prosthetics
Quality of life
silicone
Silicones
Species
Voice prostheses
Yeasts
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Summary:This study aimed to investigate the potential application of ceragenins (CSAs) as new candidacidal agents to prevent biofilm formation on voice prostheses (VPs). The deterioration of the silicone material of VPs is caused by biofilm growth on the device which leads to frequent replacement procedures and sometimes serious complications. A significant proportion of these failures is caused by Candida species. We found that CSAs have significant candidacidal activities in vitro (MIC; MFC; MBIC), and they effectively eradicate species of yeast responsible for VP failure. Additionally, in our in vitro experimental setting, when different Candida species were subjected to CSA-13 and CSA-131 during 25 passages, no tested Candida strain showed the significant development of resistance. Using liquid chromatography–mass spectrometry (LC-MS), we found that VP immersion in an ethanol solution containing CSA-131 results in silicon impregnation with CSA-131 molecules, and in vitro testing revealed that fungal biofilm formation on such VP surfaces was inhibited by embedded ceragenins. Future in vivo studies will validate the use of ceragenin-coated VP for improvement in the life quality and safety of patients after a total laryngectomy.
ISSN:2076-0817
2076-0817
DOI:10.3390/pathogens10111371