MEG3 Promotes Differentiation of Porcine Satellite Cells by Sponging miR-423-5p to Relieve Inhibiting Effect on SRF

• Published in: Cells (Basel, Switzerland) Switzerland), 2020-02, Vol.9 (2), p.449
• Main Authors: Cheng, Xiaofang, Li, Long, Shi, Gaoli, Chen, Lin, Fang, Chengchi, Li, Mengxun, Li, Changchun
• Format: Article
• Language: English
• Subjects: Animals; Cell Differentiation - physiology; Cell Proliferation - physiology; cerna; Male; meg3; MicroRNAs - genetics; MicroRNAs - metabolism; mir-423-5p; Muscle Development; myoblast differentiation; porcine satellite cells; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; Satellite Cells, Skeletal Muscle - cytology; Satellite Cells, Skeletal Muscle - metabolism; Serum Response Factor - antagonists & inhibitors; Serum Response Factor - metabolism; Swine; Transfection
• ISSN: 2073-4409; 2073-4409
• DOI: 10.3390/cells9020449

Although thousands of long noncoding RNAs (lncRNAs) have been identified in porcine growth and development, the regulation mechanisms of functional lncRNAs have not been well explored. In this study, using 5'- and 3'-rapid amplification of cDNA ends (RACE) assays, we obtained two different variants of lncRNA maternally expressed gene 3 ( ), namely, v1 and v2, that were both highly expressed in porcine skeletal muscle and in the early stage of the differentiation of porcine satellite cells. Moreover, we identified the core transcript v2. Functional analyses showed that overexpression could effectively arrest myoblasts in the G1 phase, inhibit DNA replication, and promote myoblast differentiation, whereas knockdown resulted in the opposite effects. Interestingly, the expression of serum response factor ( ), a crucial transcription factor for myogenesis process, remarkably increased and decreased in mRNA and protein levels with the respective overexpression and knockdown of . Dual luciferase reporter assay showed that could attenuate the decrease of luciferase activity of induced by miR-423-5p in a dose-dependent manner. overexpression could relieve the inhibitory effect on and myoblast differentiation induced by miR-423-5p. In addition, results of RNA immunoprecipitation analysis suggested that could act as a ceRNA for miR-423-5p. Our findings initially established a novel connection among , miR-423-5p, and in porcine satellite cell differentiation. This novel role of may shed new light on understanding of molecular regulation of lncRNA in porcine myogenesis.