Plant Tissue Trypan Blue Staining During Phytopathogen Infection

In this protocol plant tissue is stained with trypan blue dye allowing the researcher to visualize cell death. Specifically this method avoids the use of the carcinogen compound chloral hydrate, making this classical method of staining safer and faster than ever. The protocol is applied specifically...

Full description

Saved in:
Bibliographic Details
Published in:Bio-protocol 2016-12, Vol.6 (24)
Main Authors: Fernández-Bautista, Nuria, Domínguez-Núñez, José, Moreno, M. Mar, Berrocal-Lobo, Marta
Format: Article
Language:English
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this protocol plant tissue is stained with trypan blue dye allowing the researcher to visualize cell death. Specifically this method avoids the use of the carcinogen compound chloral hydrate, making this classical method of staining safer and faster than ever. The protocol is applied specifically to detect cell death on Arabidopsis leaves during the course of infection with necrotrophic fungus Botrytis cinerea.
ISSN:2331-8325
2331-8325
DOI:10.21769/BioProtoc.2078