Lipidomics of intact mitochondria by MALDI-TOF/MS

A simple and fast method of lipid analysis of isolated intact mitochondria by means of MALDI-TOF mass spectrometry is described. Mitochondria isolated from bovine heart and yeast have been employed to set up and validate the new method of lipid analysis. The mitochondrial suspension is directly appl...

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Bibliographic Details
Published in:Journal of lipid research 2012-07, Vol.53 (7), p.1417-1425
Main Authors: Angelini, Roberto, Vitale, Rita, Patil, Vinay A., Cocco, Tiziana, Ludwig, Bernd, Greenberg, Miriam L., Corcelli, Angela
Format: Article
Language:English
Subjects:
9-aminoacridine
Animals
cardiolipin
Cattle
heart
Lipids - analysis
matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
Methods
Mitochondria - chemistry
Mitochondria, Heart - chemistry
Paracoccus denitrificans
Saccharomyces cerevisiae - chemistry
Saccharomyces cerevisiae - cytology
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
yeast
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Description
Summary:A simple and fast method of lipid analysis of isolated intact mitochondria by means of MALDI-TOF mass spectrometry is described. Mitochondria isolated from bovine heart and yeast have been employed to set up and validate the new method of lipid analysis. The mitochondrial suspension is directly applied over the target and, after drying, covered by a thin layer of the 9-aminoacridine matrix solution. The lipid profiles acquired with this procedure contain all peaks previously obtained by analyzing the lipid extracts of isolated mitochondria by TLC and/or mass spectrometry. The novel procedure allows the quick, simple, precise, and accurate analysis of membrane lipids, utilizing only a tiny amount of isolated organelle; it has also been tested with intact membranes of the bacterium Paracoccus denitrificans for its evolutionary link to present-day mitochondria. The method is of general validity for the lipid analysis of other cell fractions and isolated organelles.
ISSN:0022-2275
1539-7262
DOI:10.1194/jlr.D026203