Uncoupling apical constriction from tissue invagination

Apical constriction is a widely utilized cell shape change linked to folding, bending and invagination of polarized epithelia. It remains unclear how apical constriction is regulated spatiotemporally during tissue invagination and how this cellular process contributes to tube formation in different...

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Bibliographic Details
Published in:eLife 2017-03, Vol.6
Main Authors: Chung, SeYeon, Kim, Sangjoon, Andrew, Deborah J
Format: Article
Language:English
Subjects:
Animals
apical constriction
Apoptosis
Cell Biology
Cell division
Cell size
Developmental Biology and Stem Cells
Drosophila
Drosophila - embryology
Epithelial cells
fog
fork head
Gastrulation
Genetic aspects
Insects
Internalization
Kinases
Morphogenesis
Myosin
Myosin Type II - metabolism
Physiological aspects
Rho-associated kinase
Salivary gland
Salivary Glands - embryology
Transcription factors
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Summary:Apical constriction is a widely utilized cell shape change linked to folding, bending and invagination of polarized epithelia. It remains unclear how apical constriction is regulated spatiotemporally during tissue invagination and how this cellular process contributes to tube formation in different developmental contexts. Using salivary gland (SG) invagination as a model, we show that regulation of expression by the Fork head transcription factor is required for apicomedial accumulation of Rho kinase and non-muscle myosin II, which coordinate apical constriction. We demonstrate that neither loss of spatially coordinated apical constriction nor its complete blockage prevent internalization and tube formation, although such manipulations affect the geometry of invagination. When apical constriction is disrupted, compressing force generated by a tissue-level myosin cable contributes to SG invagination. We demonstrate that fully elongated polarized SGs can form outside the embryo, suggesting that tube formation and elongation are intrinsic properties of the SG.
ISSN:2050-084X
2050-084X
DOI:10.7554/elife.22235