Assessment of Cervical Cancer Molecular-Based Screening Tools; HPV-DNA Detection versus E6/E7 mRNA Testing; First Report of a Prospective Cohort Study among Iranian Women

Human papillomavirus (HPV) has been found as the most considerable causes of cervical cancer. Recently, several molecular methods have been introduced to increase the accuracy of the screening programs and decrease the mortality rate. Among these methods, mRNA-based methods have more advantages as t...

Full description

Saved in:
Bibliographic Details
Published in:Iranian journal of public health 2020-09, Vol.49 (9), p.1734-1742
Main Authors: Mousavi, Azam-Sadat, Pouryasin, Ali, Yarandi, Fariba, Pirzadeh, Leila, Alipour, Abbas, Khodadad, Shakiba, Pouryasin, Mohammad
Format: Article
Language:English
Subjects:
Cancer
Cervical cancer
Cervix
Cohort analysis
Cytology
Deoxyribonucleic acid
DNA
Genotyping
Gynecology
Human papillomavirus
Human Papillomavirus (HPV)
Infections
Intraepithelial lesion
Malignancy
Methods
mRNA
Original
Ribonucleic acid
RNA
Sensitivity
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Human papillomavirus (HPV) has been found as the most considerable causes of cervical cancer. Recently, several molecular methods have been introduced to increase the accuracy of the screening programs and decrease the mortality rate. Among these methods, mRNA-based methods have more advantages as they assess the expression level of HPV E6 and E7 oncogenic mRNAs. This study aimed to evaluate the results of HPV RNA- and DNA-based methods among Iranian women population with normal cytology results. Overall, 4640 women were enrolled referred to the Gynecology Oncology Ward of Vali-e-Asr Hospital, private and academic clinics, Tehran, Iran from Jan 2016 to Apr 2018. To assess the HPV-DNA infection INNO-LiPA® HPV Genotyping Extra-II kit was used. For HPV-RNA assessment, Aptima HPV Assay and in house HPV-RNA genotyping methods were applied. The positivity rates of HPV infection according to DNA- and RNA-based methods were 18.0% and 11.2%, respectively (
ISSN:2251-6085
2251-6093
DOI:10.18502/ijph.v49i9.4093