Quercetin Intervention Alleviates Offspring's Oxidative Stress, Inflammation, and Tight Junction Damage in the Colon Induced by Maternal Fine Particulate Matter (PM 2.5 ) Exposure through the Reduction of Bacteroides

• Published in: Nutrients 2020-10, Vol.12 (10), p.3095
• Main Authors: Liu, Wei, Zhou, Yalin, Qin, Yong, Yu, Lanlan, Li, Ruijun, Chen, Yuhan, Xu, Yajun
• Format: Article
• Language: English
• Subjects: Abundance; air pollution; Animals; Bacteroides; Bacteroides - drug effects; Biomarkers; Catalase; Cellulose; Colon; Colon - drug effects; Colon - metabolism; Colon - pathology; Cytokines; Digestive system; Enzymes; Exposure; Female; Gastrointestinal Microbiome - drug effects; Gestation; Inflammation; Inflammation - prevention & control; Interleukin 22; intestinal barrier; Intestinal microflora; Intestine; Lactation; Male; Maternal Exposure - adverse effects; Maternal-Fetal Exchange - physiology; Metabolites; Mice, Inbred ICR; Microbiota; Offspring; Oxidation; Oxidative stress; Oxidative Stress - drug effects; Particulate matter; Particulate Matter - adverse effects; Permeability; Pregnancy; Prenatal Exposure Delayed Effects; Proteins; Quercetin; Quercetin - administration & dosage; Quercetin - pharmacology; Superoxide dismutase; Taxonomy; Tight junctions; Tight Junctions - drug effects; Tight Junctions - pathology; Zonula occludens-1 protein
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu12103095

The influences of maternal fine particulate matter (PM ) exposure on intestinal oxidative stress, inflammation, tight junctions, and gut microbiota of offspring are not well understood. Moreover, research on the dietary intervention method has not been well studied. In our study, dams received PM and quercetin intervention during gestation and lactation, and then inflammation biomarkers, oxidative stress indicators, tight junction proteins, and gut microbiota in the colon of offspring were analyzed. Compared with the control group, lower catalase (CAT) and superoxide dismutase (SOD) activities, higher interleukin-17A (IL-17A) and interleukin-22 (IL-22), decreased ZO-1 and occludin expressions, and higher abundance were observed in the offspring mice of the PM group. However, higher CAT and SOD activities, lower IL-17A and IL-22 levels, increased ZO-1 and occludin expressions, and lower abundance were found in the quercetin groups. In addition, there was a negative correlation between abundance and CAT concentration. Additionally, abundance was positively related to IL-17A and IL-22 levels. These findings suggest that maternal PM exposure may have some certain effects on intestinal oxidative stress, inflammation, and tight junctions. Quercetin administration may protect the offspring against these adverse effects. Changes of abundance play an important role in the process.