Multivessel Coronary Artery Disease Complicated by Diabetes Mellitus Has a Relatively Small Effect on Endothelial and Lipoprotein Lipases Expression in the Human Atrial Myocardium and Coronary Perivascular Adipose Tissue

• Published in: International journal of molecular sciences 2023-09, Vol.24 (17), p.13552
• Main Authors: Knapp, Małgorzata, Łukaszuk, Bartłomiej, Lisowska, Anna, Hirnle, Tomasz, Górski, Jan, Chabowski, Adrian, Mikłosz, Agnieszka
• Format: Article
• Language: English
• Subjects: Adipose tissues; Analysis; Apolipoproteins; Atherosclerosis; Blood lipids; Blood vessels; Body fat; Cardiovascular disease; Cholesterol; coronary artery disease; Coronary heart disease; Coronary vessels; Diabetes; diabetes mellitus; Diabetics; endothelial lipase; Endothelium; Enzymes; Fatty acids; Glucose; heart; High density lipoprotein; Lipids; lipoprotein lipase; Lipoproteins; Low density lipoproteins; Metabolism; Oxidative stress; Pathogenesis; Physiological aspects; Plasma; Proteins; Risk factors; Type 2 diabetes; Vein & artery diseases; Womens health
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms241713552

Endothelial (EL) and lipoprotein (LPL) lipases are enzymes involved in lipoproteins metabolism and formation of atherosclerosis, a pathological feature of coronary artery disease (CAD). This paper examines the role of the lipases in the right atrial appendage (RAA) and coronary perivascular adipose tissue (PVAT) of patients with CAD alone or with accompanying diabetes. Additionally, correlation analysis for plasma concentration of the lipases, apolipoproteins (ApoA-ApoJ) and blood lipids (Chol, HDL-C, LDL-C, TAG) was performed. We observed that CAD had little effect on the lipases gene/protein levels in the RAA, while their transcript content was elevated in the PVAT of diabetic CAD patients. Interestingly, the RAA was characterized by higher expression of EL/LPL (EL: +1-fold for mRNA, +5-fold for protein; LPL: +2.8-fold for mRNA, +12-fold for protein) compared to PVAT. Furthermore, ApoA1 plasma concentration was decreased, whereas ApoC1 and ApoH were increased in the patients with CAD and/or diabetes. The concentrations of ApoC3 and ApoD were strongly positively correlated with TAG content in the blood, and the same was true for ApoB with respect to LDL-C and total cholesterol. Although plasma concentrations of EL/LPL were elevated in the patients with diabetes, CAD alone had little effect on blood, myocardial and perivascular fat expression of the lipases.