The effect of the chitosan-collagen membrane on wound healing process in rat mandibular defect

Background: Collagen and chitosan are potential biomaterials for medical applications; chitosan-collagen membranes are used as a barrier membrane in guided tissue regeneration and guided bone regeneration. Aims: The purpose of this study was to analyze the effect of the chitosan-collagen membrane on...

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Bibliographic Details
Published in:Journal of Indian Society of Periodontology 2019-03, Vol.23 (2), p.113-118
Main Authors: Susanto, Agus, Susanah, Susi, Priosoeryanto, Bambang, Satari, Mieke, Komara, Ira
Format: Article
Language:English
Subjects:
Angiogenesis
Biological products
Biomaterials
Blood vessels
Bone growth
Chitin
Chitosan
Chitosan-collagen membrane
Collagen
Defects
Dosage and administration
Fibroblasts
Growth factors
Health aspects
Mandible
mandibular defect
Membranes
Nuclear energy
Original
Regeneration
Statistical analysis
Wound care
Wound healing
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Summary:Background: Collagen and chitosan are potential biomaterials for medical applications; chitosan-collagen membranes are used as a barrier membrane in guided tissue regeneration and guided bone regeneration. Aims: The purpose of this study was to analyze the effect of the chitosan-collagen membrane on wound healing in rat mandibular defect by counting the number of fibroblasts and new blood vessels. Materials and Methods: As much as 24 male Wistar rats were divided into two groups, the treatment and control group. Bone defects were made In the rat mandible with diamond bur with a diameter of 2 mm, then the defect was covered with a chitosan-collagen membrane, and the control group was covered without application of chitosan-collagen membrane. After the 3rd, 7th, 14th, and the 21st day, the defect site was analyzed histologically. The number of fibroblasts and blood vessels was counted under a light microscope, at five fields with ×1000 and ×400 microscope magnification. Statistical Analysis Used: This study was done by using analysis of variance and unpaired t-test. Results: The average number of fibroblasts and blood vessels in the treatment group was higher than the control group. There was a significant difference in the number of fibroblasts on the 3rd and 7th day (P = 0.001; P = 0.001) and the number of blood vessels on the 3rd day (P = 0.04). Conclusion: The chitosan-collagen membrane was able to increase the number of fibroblasts and new blood vessels in the wound healing process.
ISSN:0972-124X
0975-1580
DOI:10.4103/jisp.jisp_232_18