Early and late responses to Fusarium Head blight in durum wheat: Focus on phenylpropanoid biosynthetic pathway

• Published in: Plant stress (Amsterdam) 2024-12, Vol.14, p.100603, Article 100603
• Main Authors: Felici, Linda, Castellani, Federica, Francesconi, Sara, Vitali, Matteo, Sestili, Francesco, Balestra, Giorgio Mariano
• Format: Article
• Language: English
• Subjects: Anthocyanins; Flavonoids; Fusarium graminearum; Phenolic acids; Pigmented grains; Triticum turgidum L. subsp. Durum
• ISSN: 2667-064X; 2667-064X
• DOI: 10.1016/j.stress.2024.100603

•Phenylpropanoids pathway is early triggered in resistant genotypes.•In Purple durum the early infection increase quercetin.•Chalcone synthase gene was late boosted in stressed pigmented genotype.•Transcription factor Ppm1is late promoted by infection.•Purple durum accumulate anthocyanins due to infection. Durum wheat is among the cereal crops most susceptible to Fusarium Head Blight (FHB), a fungal disease that can lead to significant yield losses. Despite this, only limited research efforts have been directed towards understanding FHB resistance in durum wheat. Wheat grains naturally contain phenolic compounds, and anthocyanins are particularly present in the so-called pigmented wheat genotypes, such as purple pericarp ones. In this study the effects of the biotic stress caused by Fusarium graminearum infection on phenylpropanoid biosynthetic pathway in durum wheat spikes were explored, considering three genotypes with different susceptibility (including a purple pericarp genotype), and two time points (an early stage time point: 2 days post infection, and a late stage time point: 21 days post infection). At early infection stage, the F. graminearum infection triggered upregulation of all the considered genes involved in the phenylpropanoid pathway in the resistant genotype, while, in the purple pericarp genotype, the infection caused an increase in quercetin accumulation in the soluble fraction of spike extract. At late infection stage, the infection caused (in all the genotypes) a degradation of secondary cell wall and the release of the hydroxycinnamic acids esterified with arabinoxylans (ferulic acid and p-coumaric acid) and lignin-derived monomers (vanillic acid). Furthermore, chalcone synthase gene (CHS) and the transcription factor Ppm1 (Purple pericarp MYB 1) were boosted in the pigmented genotype due to infection at late infection stage. These findings contribute to the understanding of host-pathogen interactions for future breeding programs focused on improving FHB resistance in durum wheat varieties, with a particular focus on pigmented genotypes. [Display omitted]