Chemical Constituents of Callistemon subulatus and Their Anti-Pancreatic Cancer Activity against Human PANC-1 Cell Line

An n-hexane extract of Callistemon subulatus was found to exhibit potent cytotoxicity against PANC-1 human pancreatic cancer cells, preferentially under nutrition starvation conditions, with a PC50 value of 6.2 µg/mL. Phytochemical investigation of this bioactive extract resulted in the isolation of...

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Bibliographic Details
Published in:Plants (Basel) 2022-09, Vol.11 (19), p.2466
Main Authors: Maneenet, Juthamart, Tawila, Ahmed M, Omar, Ashraf M, Phan, Nguyen Duy, Ojima, Chiharu, Kuroda, Masahiro, Sato, Mao, Mizoguchi, Mio, Takahashi, Ikue, Awale, Suresh
Format: Article
Language:English
Subjects:
1-Phosphatidylinositol 3-kinase
AKT protein
anti-austerity
Antitumor agents
Autophagy
Biocompatibility
Callistemon
Callistemon subulatus
Carbon
Care and treatment
Cell death
Cell migration
Cell morphology
Composition
Cytology
Cytotoxicity
Drug development
Flavonoids
Health aspects
Hexane
Hexanes
Investigations
Lead compounds
Materia medica, Vegetable
Morphology
Myrtaceae
n-Hexane
NMR
Nuclear magnetic resonance
Nutrition
Oils & fats
Pancreatic cancer
Physiological aspects
Phytochemicals
Plant extracts
preferential cytotoxicity
TOR protein
Toxicity testing
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Summary:An n-hexane extract of Callistemon subulatus was found to exhibit potent cytotoxicity against PANC-1 human pancreatic cancer cells, preferentially under nutrition starvation conditions, with a PC50 value of 6.2 µg/mL. Phytochemical investigation of this bioactive extract resulted in the isolation of fifteen compounds (1–15), including a new compound, subulatone A (–). The structure of compound 1 was elucidated using HRFABMS and NMR spectroscopic analyses. The isolated compounds were tested for their preferential cytotoxicity against the PANC-1 human pancreatic cancer cell line, using an anti-austerity strategy. Among these, myrtucommulone A (2) showed highly potent preferential cytotoxicity, with a PC50 value of 0.28 µM. Myrtucommulone A (2) was found to alter PANC-1 cell morphology, inhibit cell migration, and downregulate the PI3K/Akt/mTOR and autophagy signaling pathways in nutrient-deprived media, leading to cancer cell death. Therefore, myrtucommulone A (2) is a lead compound for anticancer drug development based on an anti-austerity strategy.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants11192466