Function Characterization of Endogenous Plasmids in Cronobacter sakazakii and Identification of p-Coumaric Acid as Plasmid-Curing Agent

Virulence traits and antibiotic resistance are frequently provided by genes located on plasmids. However, experimental verification of the functions of these genes is often lacking due to a lack of related experimental technology. In the present study, an integrated suicide vector was used to effici...

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Published in:Frontiers in microbiology 2021-06, Vol.12, p.687243-687243
Main Authors: Ji, Xuemeng, Lu, Ping, Hu, Yaozhong, Xue, Juan, Wu, Jing, Zhang, Bowei, Zhang, Yan, Dong, Lu, Lv, Huan, Wang, Shuo
Format: Article
Language:English
Subjects:
antibiotic resistance
Cronobacter sakazakii
Microbiology
p-coumaric acid
plasmid curing
virulence
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Summary:Virulence traits and antibiotic resistance are frequently provided by genes located on plasmids. However, experimental verification of the functions of these genes is often lacking due to a lack of related experimental technology. In the present study, an integrated suicide vector was used to efficiently and specifically delete a bacterial endogenous plasmid in Cronobacter sakazakii . The pESA3 plasmid was removed from C. sakazakii BAA-894, and we confirmed that this plasmid contributes to the invasion and virulence of this strain. In addition, the pGW1 plasmid was expunged from C. sakazakii GZcsf-1, and we confirmed that this plasmid confers multidrug resistance. We further screened plasmid-curing agents and found that p -coumaric acid had a remarkable effect on the curing of pESA3 and pGW1 at sub-inhibitory concentrations. Our study investigated the contribution of endogenous plasmids pESA3 and pGW1 by constructing plasmid-cured strains using suicide vectors and suggested that p -coumaric acid can be a safe and effective plasmid-curing agent for C. sakazakii .
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2021.687243