Impact of nitrogen and phosphorus fertilizers on Cry1Ac protein contents in transgenic cotton

Abstract Application of different fertilizers to check the efficiency of expression of Bt (Bacillus thuringiensis) gene in one of the leading commercialized crops (cotton) against Lepidopteran species is of great concern. The expression of Cry protein level can be controlled by the improvement of nu...

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Bibliographic Details
Published in:Brazilian journal of biology 2023, Vol.83, p.1-7
Main Authors: Khan, S. U., Ali, S., Shah, S. H., Zia, M. A., Shoukat, S., Hussain, Z., Shahzad, A.
Format: Article
Language:English
Subjects:
Bacillus thuringiensis
BIOLOGY
Bt gene
Commercialization
Cotton
Crops
Cross-resistance
CRY protein
Cry1Ac
Cry1Ac gene
Cry1Ac toxin
Cultivars
ELISA
Enzyme-linked immunosorbent assay
fertilizer
Fertilizers
Gene expression
Insects
Nitrogen
Nutrient content
Nutrients
Pest resistance
Pests
Phosphorus
Plant resistance
Polymerase chain reaction
Proteins
Toxins
transgenic cotton
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Summary:Abstract Application of different fertilizers to check the efficiency of expression of Bt (Bacillus thuringiensis) gene in one of the leading commercialized crops (cotton) against Lepidopteran species is of great concern. The expression of Cry protein level can be controlled by the improvement of nutrients levels. Therefore, the myth of response of Cry toxin to different combinations of NP fertilizers was explored in three Bt cotton cultivars. Combinations include three levels of nitrogen and three levels of phosphorus fertilizers. Immunostrips and Cry gene(s) specific primer based PCR (Polymerase Chain Reaction) analysis were used for the presence of Bt gene that unveiled the presence of Cry1Ac gene only. Further, the ELISA (enzyme-linked immunosorbent assay) kit was used to quantify the expression of Cry1Ac protein. Under various NP fertilizers rates, the level of toxin protein exhibited highly significant differences. The highest toxin level mean was found to be 2.3740 and 2.1732 µg/g under the treatment of N150P75 kg ha-1 combination while the lowest toxin level mean was found to be 0.9158 and 0.7641 µg/g at the N50P25 kg ha-1 level at 80 and 120 DAS (Days After Sowing), respectively. It was concluded from the research that the usage of NP fertilizers has a positive relation with the expression of Cry1Ac toxin in Bt cotton. We recommend using the N150P50 kg ha-1 level as the most economical and practicable fertilizer instead of the standard dose N100P50 kg ha-1 to get the desired level of Cry1Ac level for long lasting plant resistance (
ISSN:1519-6984
1678-4375
1678-4375
DOI:10.1590/1519-6984.246436