Toxicological and Pharmacological Activities of Leptohyptis macrostachys (Benth.) Harley and J.F.B.Pastore (Lamiaceae) on Intestinal Smooth Muscle

, previously known as Benth., is used in folk medicine to relieve the symptoms of asthma, cough, and bronchitis. Recently, we showed that the ethanol extract obtained from has selective spasmolytic activity on guinea pig ileum. Therefore, the aim of this study was to characterize the spasmolytic mec...

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Published in:Frontiers in pharmacology 2020-07, Vol.11, p.1042-1042
Main Authors: de Souza, Iara Leão Luna, de Vasconcelos, Maria Allynne, da Silva, Anne Dayse Soares, Silva, Polyana Cristina Barros, Veloso, Carlos Arthur Gouveia, de Araújo, Diego Igor Alves Fernandes, Costa, Vicente Carlos de Oliveira, Pessôa, Hilzeth Luna Freire, Tavares, Josean Fechine, da Silva, Marcelo Sobral, da Silva, Bagnólia Araújo, Cavalcante, Fabiana de Andrade
Format: Article
Language:English
Subjects:
antidiarrheal activity
Lamiaceae
Leptohyptis macrostachys
Pharmacology
potassium channel
spasmolytic activity
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Summary:, previously known as Benth., is used in folk medicine to relieve the symptoms of asthma, cough, and bronchitis. Recently, we showed that the ethanol extract obtained from has selective spasmolytic activity on guinea pig ileum. Therefore, the aim of this study was to characterize the spasmolytic mechanism of this extract, investigated whether it presents toxicological and antidiarrheal activities. Therefore, the crude ethanolic extract of was analyzed by high-performance liquid chromatographic-diode array detection (HPLC-DAD). The spasmolytic effect was evaluated on guinea pig ileum, toxicological activity using rats and antidiarrheal activity using male and female mice. In HPLC-DAD analysis, Rosmarinic acid (5.44%) was the most abundant phenolic compound, being considered as a chemical marker. The spasmolytic potency of the extract on histamine-induced contraction was reduced in the presence of 1 mM TEA , a selective big-conductance K channels blocker (BK ). The extract produces a dose-dependent antidiarrheal activity, inhibiting equipotently defecation frequency and liquid stool formation. In addition, the extract has inhibited in a dose-dependent manner both castor oil-induced intestinal transit and intestinal fluid content. Thus, the spasmolytic activity of the extract involves positive modulation of BK and its antidiarrheal activity is related to inhibition of intestinal motility and secretion.
ISSN:1663-9812
1663-9812
DOI:10.3389/fphar.2020.01042