Inhibition of KCTD10 Affects Diabetic Retinopathy Progression by Reducing VEGF and Affecting Angiogenesis

Aim. We purposed to evaluate the KCTD10 effects of angiogenesis in diabetic retinopathy (DR). Methods. We induced a DR cell model using high glucose (HG) treatment of HRECs and ARPE-19 cells. A DR rat was established by injecting streptozotocin. Small interference RNA targeted KCTD10 (si-KCTD10) was...

Full description

Saved in:
Bibliographic Details
Published in:Genetics Research 2022, Vol.2022, p.4112307-10
Main Authors: Feng, Yun, Wang, Cong, Wang, Guangwei
Format: Article
Language:English
Subjects:
Angiogenesis
Animal models
Animal tissues
Animals
Apoptosis
Assaying
Cell culture
Cell cycle
Cell growth
Cell viability
Cholecystokinin
Diabetes
Diabetes mellitus
Diabetes Mellitus - metabolism
Diabetic retinopathy
Diabetic Retinopathy - genetics
Diabetic Retinopathy - metabolism
Endothelial Cells - metabolism
Enzyme-linked immunosorbent assay
Enzymes
Experiments
Glucose
Hypoxia-inducible factor 1a
Immunohistochemistry
Laboratory animals
Neovascularization, Pathologic - genetics
Neovascularization, Pathologic - metabolism
Proteins
Rats
Retina
Retina - metabolism
Retinopathy
RNA-mediated interference
siRNA
Streptozocin
Vascular endothelial growth factor
Vascular Endothelial Growth Factor A - genetics
Vascular Endothelial Growth Factor A - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Aim. We purposed to evaluate the KCTD10 effects of angiogenesis in diabetic retinopathy (DR). Methods. We induced a DR cell model using high glucose (HG) treatment of HRECs and ARPE-19 cells. A DR rat was established by injecting streptozotocin. Small interference RNA targeted KCTD10 (si-KCTD10) was used to mediate KCTD10 inhibition in cell and animal models. The roles of KCTD10 on cell viability, apoptosis, angiogenesis, and related proteins (VEGF and HIF-1α) were observed by RT-qPCR, Western blot, CCK-8 assay, TUNEL staining, tube formation assay, ELISA, and immunohistochemistry assay. Results. KCTD10 expression was upregulated in DR cells and retinal tissue of DR rats. Treatment of the cells with si-KCTD10 increased cell viability and decreased apoptosis and angiogenesis in DR cells. Inhibition of KCTD10 could reduce the expression of VEGF and HIF-1α in DR cells. Furthermore, KCTD10 inhibition reduced VEGF levels in the retinal tissue of DR rats. Conclusion. This work showed that inhibition of KCTD10 relieved angiogenesis in DR.
ISSN:1469-5073
0016-6723
1469-5073
DOI:10.1155/2022/4112307