Generation and characterization of human-derived iPSC lines from two cousins with schizophrenia and bipolar disorder and their unaffected cousin

Schizophrenia (SCZ) and bipolar disorder (BD) are debilitating neurodevelopmental disorders with high heritability. In this study, peripheral blood mononuclear cells (PBMCs) were donated by three females. An adolescent female was clinically diagnosed as first-episode SCZ. One of her cousins was clin...

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Bibliographic Details
Published in:Stem cell research 2022-08, Vol.63, p.102832-102832, Article 102832
Main Authors: Ni, Peiyan, Zhou, Chuqing, Meng, Yajing, Xue, Rui, Yang, Xia, Li, Lei, Zhao, Liansheng, Wei, Jinxue, Ni, Rongjun, Wang, Yingcheng, Ma, Xiaohong, Guo, Wanjun, Wang, Qiang, Li, Tao
Format: Article
Language:English
Subjects:
Bipolar disorder (BD)
Induced pluripotent stem cells (iPSCs)
Psychiatric disorders
Schizophrenia (SCZ)
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Summary:Schizophrenia (SCZ) and bipolar disorder (BD) are debilitating neurodevelopmental disorders with high heritability. In this study, peripheral blood mononuclear cells (PBMCs) were donated by three females. An adolescent female was clinically diagnosed as first-episode SCZ. One of her cousins was clinically diagnosed as BD and another one was unaffected control. Induced pluripotent stem cells (iPSCs) were established with reprograming factors Oct4, Sox2, Nanog, Lin28, c-myc, Klf4, and SV40LT. All lines presented normal karyotype and highly expressed pluripotency markers in vitro. All iPSCs were capable to differentiate into derivatives of three germ layers in vivo.
ISSN:1873-5061
1876-7753
DOI:10.1016/j.scr.2022.102832