Color-tunable bioluminescence imaging portfolio for cell imaging

The present study describes a color-tunable imaging portfolio together with twelve novel coelenterazine (CTZ) analogues. The three groups of CTZ analogues create diverse hues of bioluminescence (BL) ranging from blue to far red with marine luciferases. We found that the hue completes the whole color...

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Bibliographic Details
Published in:Scientific reports 2021-01, Vol.11 (1), p.2219-2219, Article 2219
Main Authors: Tamaki, Shota, Kitada, Nobuo, Kiyama, Masahiro, Fujii, Rika, Hirano, Takashi, Kim, Sung Bae, Maki, Shojiro
Format: Article
Language:English
Subjects:
631/1647
631/337
631/61
631/92
639/624
639/638
Bioluminescence
Color
Dose dependency
Humanities and Social Sciences
Hydrocarbons
Mammalian cells
multidisciplinary
Optical properties
Proteins
Science
Science (multidisciplinary)
Wavelengths
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Summary:The present study describes a color-tunable imaging portfolio together with twelve novel coelenterazine (CTZ) analogues. The three groups of CTZ analogues create diverse hues of bioluminescence (BL) ranging from blue to far red with marine luciferases. We found that the hue completes the whole color palette in the visible region and shows red-shifted BL with a marine luciferase: for example, Renilla luciferase 8 (RLuc8) and Artificial Luciferase 16 (ALuc16) show 187 nm- and 105 nm-redshifted spectra, respectively, by simply replacing the substrate CTZ with 1d . The optical properties of the new CTZ analogues were investigated such as the kinetic parameters, dose dependency, and luciferase specificity. The 2-series CTZ analogues interestingly have specificity to ALucs and are completely dark with RLuc derivatives, and 3d is highly specific to only NanoLuc. We further determined the theoretical background of the red-shifted BL maximum wavelengths ( λ BL ) values according to the extended π conjugation of the CTZ backbone using Density Functional Theory (DFT) calculations. This color-tunable BL imaging system provides a useful multicolor imaging portfolio that efficiently images molecular events in mammalian cells.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-021-81430-1