Insights of Phenolic Pathway in Fruits: Transcriptional and Metabolic Profiling in Apricot ( Prunus armeniaca )

• Published in: International journal of molecular sciences 2021-03, Vol.22 (7), p.3411
• Main Authors: Gómez-Martínez, Helena, Gil-Muñoz, Francisco, Bermejo, Almudena, Zuriaga, Elena, Badenes, Maria L
• Format: Article
• Language: English
• Subjects: Acids; Alcohol Oxidoreductases - genetics; Alcohol Oxidoreductases - metabolism; Ammonia; Anthocyanins; Antioxidants; Apricots; Biosynthesis; Critical point; DFR; Enzymes; Flavonoids; Flavonols; FLS; Food quality; Fruits; Gene expression; Genes; High-performance liquid chromatography; Metabolism; Metabolites; Metabolome; Oxidoreductases - genetics; Oxidoreductases - metabolism; PAL; Phenolic compounds; phenolic pathway; Phenols; Phenylalanine; Phenylalanine ammonia-lyase; Phenylalanine Ammonia-Lyase - genetics; Phenylalanine Ammonia-Lyase - metabolism; Phylogenetics; Plant Proteins - genetics; Plant Proteins - metabolism; Polyphenols; Polyphenols - biosynthesis; Polyphenols - genetics; Proteins; Prunus - genetics; Prunus - metabolism; Prunus armeniaca; Quality management; Reductases; Secondary metabolites; Transcription; Transcriptome
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms22073411

There is an increasing interest in polyphenols, plant secondary metabolites, in terms of fruit quality and diet, mainly due to their antioxidant effect. However, the identification of key gene enzymes and their roles in the phenylpropanoid pathway in temperate fruits species remains uncertain. Apricot ( ) is a Mediterranean fruit with high diversity and fruit quality properties, being an excellent source of polyphenol compounds. For a better understanding of the phenolic pathway in these fruits, we selected a set of accessions with genetic-based differences in phenolic compounds accumulation. HPLC analysis of the main phenolic compounds and transcriptional analysis of the genes involved in key steps of the polyphenol network were carried out. Phenylalanine ammonia-lyase (PAL), dihydroflavonol-4-reductase (DFR) and flavonol synthase (FLS) were the key enzymes selected. Orthologous of the genes involved in transcription of these enzymes were identified in apricot: , , , and . Transcriptional data of the genes involved in those critical points and their relationships with the polyphenol compounds were analyzed. Higher expression of and has been associated with red-blushed accessions. Differences in expression between paralogues could be related to the presence of a BOXCOREDCPAL cis-acting element related to the genes involved in anthocyanin synthesis , and .