Effect of submucosal cryotherapy compared with steroids and NSAIDs injections on Substance P and Interleukin 6 pulpal release in experimentally induced pulpal inflammation in rabbits

To compare the effect of submucosal cryotherapy using cold saline to dexamethasone sodium phosphate and diclofenac sodium injections on substance P and interleukin 6 release in experimentally induced pulpal inflammation in rabbits' molar teeth. Fifteen rabbits were randomly classified into 3 gr...

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Bibliographic Details
Published in:Journal of applied oral science 2024-01, Vol.32, p.e20240017
Main Authors: Shalabi, Mai, Mahran, Abeer H, Elsewif, Tarek
Format: Article
Language:English
Subjects:
Analgesics
Animal model
Animals
Anti-Inflammatory Agents - pharmacology
Anti-Inflammatory Agents, Non-Steroidal - pharmacology
Cryotherapy
Cryotherapy - methods
Dental Pulp - drug effects
DENTISTRY, ORAL SURGERY & MEDICINE
Dexamethasone - analogs & derivatives
Dexamethasone - pharmacology
Diclofenac - pharmacology
Disease Models, Animal
Enzyme-Linked Immunosorbent Assay
Inflammation
Interleukin 6
Interleukin-6 - analysis
Male
Original
Pulpitis - therapy
Rabbits
Random Allocation
Reference Values
Reproducibility of Results
Saline Solution
Statistics, Nonparametric
Substance P
Substance P - analysis
Time Factors
Treatment Outcome
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Summary:To compare the effect of submucosal cryotherapy using cold saline to dexamethasone sodium phosphate and diclofenac sodium injections on substance P and interleukin 6 release in experimentally induced pulpal inflammation in rabbits' molar teeth. Fifteen rabbits were randomly classified into 3 groups according to the submucosal injection given: cold saline, dexamethasone sodium phosphate, and diclofenac sodium. A split-mouth design was adopted, the right mandibular molars were experimental, and the left molars served as the control without injections. Intentional pulp exposures were created and left for 6 hours to induce pulpitis. Pulpal tissue was extracted and examined for SP and IL-6 levels using ELISA. Within each group, the level of cytokines released was measured for both control and experimental groups for intragroup comparison to determine the effect of injection. The percentage reduction of each mediator was calculated compared with the control side for intergroup comparison then the correlation between SP and IL-6 levels was analyzed using Spearman's rank order correlation coefficient. Statistical analysis was performed, and the significance level was set at p
ISSN:1678-7757
1678-7765
1678-7765
DOI:10.1590/1678-7757-2024-0017