Targeted regulation of 5-aminolevulinic acid enhances flavonoids, anthocyanins and proanthocyanidins accumulation in Vitis davidii callus

• Published in: BMC plant biology 2024-10, Vol.24 (1), p.944-15, Article 944
• Main Authors: Lai, Chengchun, Zhang, Jing, Lai, Gongti, He, Liyuan, Xu, Heng, Li, Siyu, Che, Jianmei, Wang, Qi, Guan, Xuefang, Huang, Juqing, Lai, Pufu, Chen, Guixin
• Format: Article
• Language: English
• Subjects: 5-aminolevulinic acid; Accumulation; Acids; Aminolevulinic acid; Aminolevulinic Acid - metabolism; Analysis; Anthocyanins; Anthocyanins - metabolism; Biosynthesis; Callus; Callus culture; Cell culture; Diseases and pests; Flavonoids; Flavonoids - metabolism; Food industry; Food quality; Food sources; Fruits; Gene expression; Gene Expression Regulation, Plant; Genes; Genetic aspects; Grapes; Growth; Mass spectrometry; Metabolism; Metabolites; Plant growth; Plant layout; Plant metabolites; Proanthocyanidins; Proanthocyanidins - metabolism; Regression analysis; Scientific imaging; Spine; Spine grape; Vitis - drug effects; Vitis - genetics; Vitis - metabolism; Vitis davidii
• ISSN: 1471-2229; 1471-2229
• DOI: 10.1186/s12870-024-05667-4

Spine grape (Vitis davidii) is a promising source of high-quality anthocyanins, with vast potential for application in food, pharmaceutical, and cosmetic industries. However, their availability is limited by resource constraints. Plant cell culture has emerged as a valuable approach for anthocyanin production and serves as an ideal model to investigate the regulation of anthocyanin biosynthesis. Elicitors are employed to achieve targeted enhancement of anthocyanin biosynthesis. The present study investigated the impact of 5-aminolevulinic acid (ALA) as an elicitor on the accumulation of anthocyanins and flavonoids during spine grape callus growth. Specifically, we examined the effects of ALA on anthocyanin and its component accumulation in callus, and biosynthetic anthocyanin gene expression. ALA at 25 µg/L increased the biomass of spine grape callus. ALA induction enhanced the levels of flavonoids, anthocyanins and proanthocyanidins in callus, with maximum values reaching 911.11 mg/100 g DW, 604.60 mg/100 g DW, and 5357.00 mg/100 g DW, respectively, after callus culture for 45 days. Notably, those levels were 1.47-, 1.93- and 1.83-fold higher than controls. ALA induction modulated the flavonoid profile, and among 97 differential flavonoid metabolites differing from controls, 77 were upregulated and 20 were downregulated. Six kinds of anthocyanins, namely cyanidin (8), delphinidin (6), peonidin (5), malvidin (4), petunidin (3) and pelargonidin (3), were detected in callus, with peonidin most abundant. Compared with controls, anthocyanin components were increased in ALA-treated callus. The key genes PAL1, PAL2, PAL4, CHI, CHS3, F3'H, F3H, FLS, DFR, UFGT, MYBA1, LDOX, OMT3, GT1 and ACT involved in anthocyanin biosynthesis were upregulated following ALA treatment, resulting in anthocyanin accumulation. This study revealed a novel mode of ALA-mediated promotion of plant anthocyanin biosynthesis and accumulation at the cellular level, and a strategy for enhancing anthocyanin content in spine grape callus. The findings advance commercial-scale production of anthocyanins via spine grape callus culture. we also explored the accumulation patterns of flavonoids and anthocyanins under ALA treatment. Augmentation of anthocyanins coincided with elevated expression levels of most genes involved in anthocyanin biosynthesis within spine grape callus following ALA treatment.