Apoptotic Induction in Human Cancer Cell Lines by Antimicrobial Compounds from Antarctic Streptomyces fildesensis (INACH3013)

The Antarctic Streptomyces fildesensis has been recognized for its production of antimicrobial compounds with interesting biological activities against foodborne bacteria and multi-resistant strains, but not for its potential antiproliferative activity and mechanisms involved. Two bioactive ethyl ac...

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Bibliographic Details
Published in:Fermentation (Basel) 2023-02, Vol.9 (2), p.129
Main Authors: Astudillo-Barraza, David, Oses, Romulo, Henríquez-Castillo, Carlos, Vui Ling Wong, Clemente Michael, Pérez-Donoso, José M., Purcarea, Cristina, Fukumasu, Heidge, Fierro-Vásquez, Natalia, Pérez, Pablo A., Lavin, Paris
Format: Article
Language:English
Subjects:
Acetic acid
Antibiotics
anticancer
antimicrobial
Antimicrobial activity
Antimicrobial agents
Apoptosis
Breast cancer
Caspase
Chromatography
Colon cancer
Cytology
cytotoxic
Cytotoxicity
Epithelial cells
Ethyl acetate
Genomes
High-performance liquid chromatography
Membrane potential
Metabolites
Mitochondria
mitochondrial membrane permeability
Pathogens
Prostate
Prostate cancer
RNA polymerase
Streptomyces
Streptomyces fildesensis
Thin-layer chromatography
Tumor cell lines
Ultraviolet radiation
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Summary:The Antarctic Streptomyces fildesensis has been recognized for its production of antimicrobial compounds with interesting biological activities against foodborne bacteria and multi-resistant strains, but not for its potential antiproliferative activity and mechanisms involved. Two bioactive ethyl acetate extract (EAE) fractions were purified via thin-layer chromatography and High-Performance Liquid Chromatography (HPLC), showing that orange-colored compounds displayed antimicrobial activity against pathogenic bacteria even after shock thermal treatment. The UV–VIS features of the active compounds, the TLC assay with actinomycin-D pure standard, Fourier transform infrared (FTIR) spectra and the ANTISMASH analysis support the presence of actinomycin-like compounds. We demonstrated that S. fildesensis displays antiproliferative activity against human tumor cell lines, including human breast cancer (MCF-7), prostate cancer (PC-3), colon cancer (HT-29) and non-tumoral colon epithelial cells (CoN). The half-maximal effective concentrations (EC50) ranged from 3.98 µg/mL to 0.1 µg/mL. Our results reveal that actinomycin-like compounds of S. fildesensis induced apoptosis mediated by caspase activation, decreasing the mitochondrial membrane potential and altering the cell morphology in all tumoral and non-tumoral cell lines analyzed. These findings confirm the potential of the psychrotolerant Antarctic S. fildesensis species as a promising source for obtaining potential novel anticancer compounds.
ISSN:2311-5637
2311-5637
DOI:10.3390/fermentation9020129