Multi-omics profiling reveals rhythmic liver function shaped by meal timing

Post-translational modifications (PTMs) couple feed-fast cycles to diurnal rhythms. However, it remains largely uncharacterized whether and how meal timing organizes diurnal rhythms beyond the transcriptome. Here, we systematically profile the daily rhythms of the proteome, four PTMs (phosphorylatio...

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Bibliographic Details
Published in:Nature communications 2023-09, Vol.14 (1), p.6086-6086, Article 6086
Main Authors: Huang, Rongfeng, Chen, Jianghui, Zhou, Meiyu, Xin, Haoran, Lam, Sin Man, Jiang, Xiaoqing, Li, Jie, Deng, Fang, Shui, Guanghou, Zhang, Zhihui, Li, Min-Dian
Format: Article
Language:English
Subjects:
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Circadian rhythms
Diurnal
Fatty acids
Glycosylation
Humanities and Social Sciences
Liver
multidisciplinary
Nutrient availability
Period 2 protein
Phosphorylation
Post-translation
Proteomes
Proteomics
Rhythm
Science
Science (multidisciplinary)
Sleep and wakefulness
Transcriptomes
Translation
Ubiquitin
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Summary:Post-translational modifications (PTMs) couple feed-fast cycles to diurnal rhythms. However, it remains largely uncharacterized whether and how meal timing organizes diurnal rhythms beyond the transcriptome. Here, we systematically profile the daily rhythms of the proteome, four PTMs (phosphorylation, ubiquitylation, succinylation and N-glycosylation) and the lipidome in the liver from young female mice subjected to either day/sleep time-restricted feeding (DRF) or night/wake time-restricted feeding (NRF). We detect robust daily rhythms among different layers of omics with phosphorylation the most nutrient-responsive and succinylation the least. Integrative analyses reveal that clock regulation of fatty acid metabolism represents a key diurnal feature that is reset by meal timing, as indicated by the rhythmic phosphorylation of the circadian repressor PERIOD2 at Ser971 (PER2-pSer971). We confirm that PER2-pSer971 is activated by nutrient availability in vivo. Together, this dataset represents a comprehensive resource detailing the proteomic and lipidomic responses by the liver to alterations in meal timing. Post-translational modifications (PTMs) couple feed-fast cycles to circadian clocks. Here, the authors systematically profile daily rhythms of the proteome, 4 PTMs and lipidome in mouse livers under TRF, providing a comprehensive resource detailing rhythmic liver functions shaped by meal timing.
ISSN:2041-1723
2041-1723
DOI:10.1038/s41467-023-41759-9