Ultrastructural Features of Gold Nanoparticles Interaction with HepG2 and HEK293 Cells in Monolayer and Spheroids

Use of multicellular spheroids in studies of nanoparticles (NPs) has increased in the last decade, however details of NPs interaction with spheroids are poorly known. We synthesized AuNPs (12.0 ± 0.1 nm in diameter, transmission electron microscopy (TEM data) and covered them with bovine serum album...

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Bibliographic Details
Published in:Nanomaterials (Basel, Switzerland) Switzerland), 2020-10, Vol.10 (10), p.2040
Main Authors: Chelobanov, Boris, Poletaeva, Julia, Epanchintseva, Anna, Tupitsyna, Anastasiya, Pyshnaya, Inna, Ryabchikova, Elena
Format: Article
Language:English
Subjects:
AuBSA-NPs
AuNPs
AuPEI-NPs
Bovine serum albumin
Cell culture
Cell lines
Chemical compounds
Electron microscopy
Embryos
Gold
Hepatocellular carcinoma
Laboratory animals
Microscopy
Monolayers
Morphology
Nanoparticles
Polyethyleneimine
Scanning electron microscopy
Serum albumin
Spectrum analysis
Spheroids
Transmission electron microscopy
ultrastructure of HEK293 cells and spheroids
ultrastructure of HepG2 cells and spheroids
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Summary:Use of multicellular spheroids in studies of nanoparticles (NPs) has increased in the last decade, however details of NPs interaction with spheroids are poorly known. We synthesized AuNPs (12.0 ± 0.1 nm in diameter, transmission electron microscopy (TEM data) and covered them with bovine serum albumin (BSA) and polyethyleneimine (PEI). Values of hydrodynamic diameter were 17.4 ± 0.4; 35.9 ± 0.5 and ±125.9 ± 2.8 nm for AuNPs, AuBSA-NPs and AuPEI-NPs, and Z-potential (net charge) values were −33.6 ± 2.0; −35.7 ± 1.8 and 39.9 ± 1.3 mV, respectively. Spheroids of human hepatocarcinoma (HepG2) and human embryo kidney (HEK293) cells (Corning ® spheroid microplates CLS4515-5EA), and monolayers of these cell lines were incubated with all NPs for 15 min–4 h, and fixed in 4% paraformaldehyde solution. Samples were examined using transmission and scanning electron microscopy. HepG2 and HEK2893 spheroids showed tissue-specific features and contacted with culture medium by basal plasma membrane of the cells. HepG2 cells both in monolayer and spheroids did not uptake of the AuNPs, while AuBSA-NPs and AuPEI-NPs readily penetrated these cells. All studied NPs penetrated HEK293 cells in both monolayer and spheroids. Thus, two different cell cultures maintained a type of the interaction with NPs in monolayer and spheroid forms, which not depended on NPs Z-potential and size.
ISSN:2079-4991
2079-4991
DOI:10.3390/nano10102040