Production of citric acid by Aspergillus niger immobilized in Detarium microcarpum matrix

Aims: This study investigated the potential use of Detarium microcarpum as a support matrix for the immobilization offungal cell and its subsequent use in the production of citric acid.Methodology and results: Conidia of Aspergillus niger were immobilized on Detarium microcarpum matrix crosslinkedwi...

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Bibliographic Details
Published in:Malaysian Journal of Microbiology 2013-06, Vol.9 (2), p.161-165
Main Authors: Kareem, S. O., Oladipupo, I. O., Omemu, A. M., Babajide, J. M.
Format: Article
Language:English
Subjects:
Aspergillus niger
Cassava whey
Detarium microcarpum
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Summary:Aims: This study investigated the potential use of Detarium microcarpum as a support matrix for the immobilization offungal cell and its subsequent use in the production of citric acid.Methodology and results: Conidia of Aspergillus niger were immobilized on Detarium microcarpum matrix crosslinkedwith glutaraldehyde (2.5% v/v). Effects of various immobilized conditions on citric acid yield were determined. Citric acidproduction was optimum at 9% gel concentration, 5.0 mm bead size and 400 mg spore load. Higher citric acid yield (82g/L) was obtained by immobilized cells while free cells gave (61 g/L). Immobilized gels were stable for 15 days in thefermentation medium and retained about 75% of initial yield after five repeated cycles.Conclusion, significance and impact of study: This study therefore shows that D. microcarpum is a novel matrix forcell immobilization and will enhance production of citric acid in batch system.
ISSN:2231-7538
1823-8262
2231-7538
DOI:10.21161/mjm.47212