Host influence on the eukaryotic virome of sympatric mosquitoes and abundance of diverse viruses with a broad host range

• Published in: PloS one 2024-04, Vol.19 (4), p.e0300915-e0300915
• Main Authors: Morel, Côme, Gil, Patricia, Exbrayat, Antoni, Loire, Etienne, Charriat, Florian, Prepoint, Baptiste, Condachou, Celine, Gimonneau, Geoffrey, Fall, Assane Gueye, Biteye, Biram, Seck, Momar Talla, Eloit, Marc, Gutierrez, Serafin
• Format: Article
• Language: English
• Subjects: Aedes - virology; Analysis; Animals; Biological research; Biology and Life Sciences; Biology, Experimental; Computer and Information Sciences; Culex - virology; Culicidae - virology; Disease transmission; Distribution; Earth Sciences; Ecology; Ecology and Environmental Sciences; Ecosystem; Genetic aspects; Genotype; Health aspects; Host Specificity; Medicine and Health Sciences; Mosquito Vectors - virology; Mosquitoes; Physiological aspects; Sympatry; Virome - genetics; Virus-vector relationships; Viruses - classification; Viruses - genetics; Viruses - isolation & purification
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0300915

Mosquitoes harbor a large diversity of eukaryotic viruses. Those viromes probably influence mosquito physiology and the transmission of human pathogens. Nevertheless, their ecology remains largely unstudied. Here, we address two key questions in virome ecology. First, we assessed the influence of mosquito species on virome taxonomic diversity and relative abundance. Contrary to most previous studies, the potential effect of the habitat was explicitly included. Thousands of individuals of Culex poicilipes and Culex tritaeniorhynchus, two vectors of viral diseases, were concomitantly sampled in three habitats over two years. A total of 95 viral taxa from 25 families were identified with meta-transcriptomics, with 75% of taxa shared by both mosquitoes. Viromes significantly differed by mosquito species but not by habitat. Differences were largely due to changes in relative abundance of shared taxa. Then, we studied the diversity of viruses with a broad host range. We searched for viral taxa shared by the two Culex species and Aedes vexans, another disease vector, present in one of the habitats. Twenty-six out of the 163 viral taxa were found in the three mosquitoes. These taxa encompassed 14 families. A database analysis supported broad host ranges for many of those viruses, as well as a widespread geographical distribution. Thus, the viromes of mosquitoes from the same genera mainly differed in the relative abundance of shared taxa, whereas differences in viral diversity dominated between mosquito genera. Whether this new model of virome diversity and structure applies to other mosquito communities remains to be determined.