Bioactive constituents of n-butanol subfractions of Adansonia digitata and Corchorus olitorius against gamma-irradiation-induced hepatic damage, lipid profile and antioxidant alterations in rats

• Published in: Food chemistry advances 2024-06, Vol.4, p.100528, Article 100528
• Main Authors: Bello, Kabir O., Oloyede, Hussein O.B., Salawu, Musa O., Yakubu, Musa T.
• Format: Article
• Language: English
• Subjects: Adansonia digitata; Antioxidant; Cellular alterations; Corchorus olitorius; Hepatic damage; γ-irradiation
• ISSN: 2772-753X; 2772-753X
• DOI: 10.1016/j.focha.2023.100528

•The rats were exposed to 6 grey gamma irradiation.•The exposure significantly altered cellular alteration such as antioxidant, lipid profile and hepatocyte.•The HPLC fingerprinting of the n-butanol subfractions of A. digitata and C. olitorius revealed the presence of bioactive constituents such as catechin, luteolin, astragalin, and quercetin.•The bioactive constituents of the subfractions of A. digitata and C. olitorius have significantly restored the cellular alterations observed in the rats exposed to 6 grey gamma irradiation. Rats’ exposure to radiation led to an increased free radical from sub-toxic (24 µg/mol) to toxic concentration (120 µg/mol) in the system of rats. Free radical level exacerbate oxidative stress, thus, leading to hepatic damage, lipid profile and antioxidant alterations. This study investigated the effects of the bioactive constituent of n-butanol subfractions (subf) 1, 2, 3 and 4 of Adansonia digitata (A. digitata) and 1, 2 and 3 of Corchorus olitorius (C. olitorius) leaves on irradiation-induced hepatic damage, lipid profile and antioxidant alterations in system of Rats. A total of 54 rats (198 ± 5.00 g) were distributed into 9 groups of 6 each. Group I were fed with rat chow and distilled water only, all other groups were exposed to a single dose of 6 grey gamma irradiation, such that groups II, III, IV, V, VI, VII, VIII and IX were administered distilled water only, 1000 mg/kg body weight subfractions 1, 2, 3 and 4 of A. digitata, subfraction 1, 2 and 3 of C. olitorius respectively for 7 days. HPLC fingerprinting of the bioactive constituents of the subf of A. digitata and C. olitorius revealed the presence of catechin, eleagnin, quercetin, epicatechin, epigallocatechin, luteolin, astragalin, tocopherol, quercetin and catechin respectively. Irradiation significantly (p