Immobilization as a strategy for improving enzyme properties-application to oxidoreductases

The main objective of the immobilization of enzymes is to enhance the economics of biocatalytic processes. Immobilization allows one to re-use the enzyme for an extended period of time and enables easier separation of the catalyst from the product. Additionally, immobilization improves many properti...

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Bibliographic Details
Published in:Molecules 2014-06, Vol.19 (7), p.8995-9018
Main Authors: Guzik, Urszula, Hupert-Kocurek, Katarzyna, Wojcieszyńska, Danuta
Format: Article
Language:English
Subjects:
Animals
Biocatalysis
Biodegradation, Environmental
Biotechnology
Enzyme Stability
Enzymes, Immobilized - chemistry
Humans
immobilization
oxidoreductases
Oxidoreductases - chemistry
Review
stability of enzyme
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Summary:The main objective of the immobilization of enzymes is to enhance the economics of biocatalytic processes. Immobilization allows one to re-use the enzyme for an extended period of time and enables easier separation of the catalyst from the product. Additionally, immobilization improves many properties of enzymes such as performance in organic solvents, pH tolerance, heat stability or the functional stability. Increasing the structural rigidity of the protein and stabilization of multimeric enzymes which prevents dissociation-related inactivation. In the last decade, several papers about immobilization methods have been published. In our work, we present a relation between the influence of immobilization on the improvement of the properties of selected oxidoreductases and their commercial value. We also present our view on the role that different immobilization methods play in the reduction of enzyme inhibition during biotechnological processes.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules19078995