Alteration of functional connectivity in the cortex and major brain networks of non-human primates following focused ultrasound exposure in the dorsal striatum

• Published in: Brain stimulation 2023-07, Vol.16 (4), p.1196-1204
• Main Authors: Liu, Dong, Munoz, Fabian, Sanatkhani, Soroosh, Pouliopoulos, Antonios N, Konofagou, Elisa E, Grinband, Jack, Ferrera, Vincent P
• Format: Article
• Language: English
• Subjects: Animals; Blood-Brain Barrier; Blood-brain barrier opening; Brain - diagnostic imaging; Brain - physiology; Brain networks; Cerebral Cortex - diagnostic imaging; Focused ultrasound; Functional connectivity; Humans; Magnetic Resonance Imaging; Microbubbles; Neuromodulation; Primates; Resting state functional MRI
• ISSN: 1935-861X; 1876-4754
• DOI: 10.1016/j.brs.2023.08.003

Focused ultrasound (FUS) is a non-invasive neuromodulation technology that is being investigated for potential treatment of neurological and psychiatric disorders. FUS combined with microbubbles can temporarily open the intact blood-brain barrier (BBB) of animals and humans, and facilitate drug delivery. FUS exposure, either with or without microbubbles, has been demonstrated to alter the behavior of non-human primates (NHP), and previous studies have demonstrated the transient and long-term effects of FUS neuromodulation on functional connectivity using resting state functional MRI. The behavioral effects of FUS vary depending on whether or not it is applied in conjunction with microbubbles to open the BBB, but it is unknown whether opening the BBB affects functional connectivity differently than FUS alone. To compare the effects of applying FUS alone (FUS neuromodulation) and FUS with microbubbles (FUS-BBB opening) on changes of resting state functional connectivity in NHP. We applied 2 min FUS exposure without (neuromodulation) and with microbubbles (BBB opening) in the dorsal striatum of lightly anesthetized non-human primates, and acquired resting state functional MRI 40 min respectively after FUS exposure. The functional connectivity (FC) in the cortex and major brain networks between the two approaches were measured and compared. When applying FUS exposure to the caudate nucleus of NHP, we found that both FUS neuromodulation can activate FC between caudate and insular cortex, while inhibiting the FC between caudate and motor cortex. FUS-BBB opening can activate FC between the caudate and medial prefrontal cortex, and within the frontotemporal network (FTN). We also found both FUS and FUS-BBB opening can significantly activate FC within the default mode network (DMN). The results suggest applying FUS to a deep brain structure can alter functional connectivity in the DMN and FTN, and that FUS neuromodulation and FUS-mediated BBB opening can have different effects on patterns of functional connectivity.