Optimisation of Simultaneous Saccharification and Fermentation (SSF) for Biobutanol Production Using Pretreated Oil Palm Empty Fruit Bunch

• Published in: Molecules (Basel, Switzerland) Switzerland), 2018-08, Vol.23 (8), p.1944
• Main Authors: Md Razali, Nur Atheera Aiza, Ibrahim, Mohamad Faizal, Kamal Bahrin, Ezyana, Abd-Aziz, Suraini
• Format: Article
• Language: English
• Subjects: biobutanol; Bioengineering; Bioprocessing; Butanols - metabolism; Cellulase; Cellulase - metabolism; Clostridium acetobutylicum - metabolism; Clostridium acetobutylicum ATCC 824; Design optimization; Fermentation; Fruit - metabolism; Fruits; Hydrogen-Ion Concentration; Hydrolysis; oil palm empty fruit bunch (OPEFB); optimisation; Palm Oil; Response surface methodology; Saccharification; simultaneous saccharification and fermentation (SSF); Substrates; Sugars - metabolism; Temperature
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules23081944

This study was conducted in order to optimise simultaneous saccharification and fermentation (SSF) for biobutanol production from a pretreated oil palm empty fruit bunch (OPEFB) by ATCC 824. Temperature, initial pH, cellulase loading and substrate concentration were screened using one factor at a time (OFAT) and further statistically optimised by central composite design (CCD) using the response surface methodology (RSM) approach. Approximately 2.47 g/L of biobutanol concentration and 0.10 g/g of biobutanol yield were obtained after being screened through OFAT with 29.55% increment (1.42 fold). The optimised conditions for SSF after CCD were: temperature of 35 °C, initial pH of 5.5, cellulase loading of 15 FPU/g-substrate and substrate concentration of 5% ( / ). This optimisation study resulted in 55.95% increment (2.14 fold) of biobutanol concentration equivalent to 3.97 g/L and biobutanol yield of 0.16 g/g. The model and optimisation design obtained from this study are important for further improvement of biobutanol production, especially in consolidated bioprocessing technology.