Desenvolvimento de um método para a determinação de tioconazol associado a nanocápsulas poliméricas por cromatografia líquida

The aim of this work was to develop and validate an analytical method for the quantification of tioconazole in polymeric nanocapsule suspensions by high performance liquid chromatography with UV detection. The analysis was performed with a mobile phase composed of methanol:water (80:20) and 0.18% am...

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Bibliographic Details
Published in:Química Nova 2014-07, Vol.37 (6), p.996-999
Main Authors: Andréia P. G. Härter, Mariana H. Motta, Júlia G. de Barros, Roseane F. Ribeiro, Andréa I. H. Adams, Scheila R. Schaffazick, Cristiane de B. da Silva
Format: Article
Language:English
Subjects:
polymeric nanocapsules
tioconazole
validation
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Summary:The aim of this work was to develop and validate an analytical method for the quantification of tioconazole in polymeric nanocapsule suspensions by high performance liquid chromatography with UV detection. The analysis was performed with a mobile phase composed of methanol:water (80:20) and 0.18% ammonium hydroxide; RP-18 column and UV detection at 219 nm. The method proved to be linear in the concentration range of 5-50 µg mL-1 (r = 0.9999), specific, precise (repeatability RSD = 1.42%, intermediate precision RSD = 1.17%), accurate (98 - 102%) and robust (RSD < 2.0%). In conclusion, a simple and rapid method was validated proving suitable for quantification of tioconazole in polymeric nanocapsules.
ISSN:1678-7064
DOI:10.5935/0100-4042.20140153