Myricanol 5-fluorobenzyloxy ether regulation of survivin pathway inhibits human lung adenocarcinoma A549 cells growth in vitro

• Published in: BMC complementary and alternative medicine 2020-09, Vol.20 (1), p.269-269, Article 269
• Main Authors: Dai, Guan-Hai, Chen, Xuan, Ren, Ze-Ming, Dai, Chen-Jie, Tong, Ye-Ling, Chai, Ke-Qun
• Format: Article
• Language: English
• Subjects: A549 Cells; Adenocarcinoma; Adenocarcinoma of Lung - drug therapy; Anticancer properties; Antineoplastic Agents - pharmacology; Antitumor; Antitumor agents; Apoptosis; Bcl-2 protein; Biotechnology industries; Cancer therapies; Caspase-9; Cell cycle; Cell Cycle - drug effects; Cell growth; Cell growth inhibition; Cell Line, Tumor; Cell migration; Cell Movement - drug effects; Cell Proliferation - drug effects; Chemical modification; Chemotherapy; Cloning; Colonies; Cytotoxicity; Diarylheptanoids - pharmacology; Down-Regulation - drug effects; Drug resistance; Experiments; Gene expression; Genes; Growth; Human lung adenocarcinoma A549; Humans; In vitro methods and tests; Lung cancer; Lung Neoplasms - drug therapy; Lungs; Medical research; Membrane potential; Membrane Potential, Mitochondrial - drug effects; Mitochondria; Mitochondrial membrane potential; Myricanol 5-fluorobenzyloxy ether; Penicillin; Poly(ADP-ribose) polymerase; Proteins; Scratch tests; Survival; Survivin; Survivin - drug effects; Survivin pathway; Toxicity; Tumors; Up-Regulation - drug effects; Vectors (Biology)
• ISSN: 2662-7671; 2662-7671; 1472-6882
• DOI: 10.1186/s12906-020-03062-8

This study aimed to explore the growth inhibitory effect of myricanol 5-fluorobenzyloxy ether (5FEM) and its underlying mechanisms in human lung adenocarcinoma A549 cells in vitro. 5FEM was obtained by the chemical modification of myricanol with fluorobenzyloxy ether at the OH(5) position. The cytotoxicity, cell apoptosis, cell cycle, mitochondrial membrane potential (ΔΨm), scratch test, colony formation, and the expression levels of the key survivin pathway-related genes in A549 were evaluated. 5FEM could significantly inhibit A549 cell growth; induce cell apoptosis; increase G0/G1 population; reduce ΔΨm; inhibit cell migration and colony formation; upregulate caspase-9, P21, and Bax expression levels; and downregulate PARP, survivin, and Bcl-2 expression level. These results enhanced our understanding of 5FEM and aid the discovery of novel myricanol derivatives as potential antitumor agents.