Design, Synthesis, Characterization, and In Vitro Evaluation of a New Cross-Linked Hyaluronic Acid for Pharmaceutical and Cosmetic Applications

Hyaluronic acid (HA), an excellent biomaterial with unique bio properties, is currently one of the most interesting polymers for many biomedical and cosmetic applications. However, several of its potential benefits are limited as it is rapidly degraded by hyaluronidase enzymes. To improve the half-l...

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Bibliographic Details
Published in:Pharmaceutics 2021-10, Vol.13 (10), p.1672
Main Authors: Sciabica, Sabrina, Tafuro, Giovanni, Semenzato, Alessandra, Traini, Daniela, Silva, Dina M., Reis, Larissa Gomes Dos, Canilli, Luisa, Terno, Massimo, Durini, Elisa, Vertuani, Silvia, Baldisserotto, Anna, Manfredini, Stefano
Format: Article
Language:English
Subjects:
Biocompatibility
cross-linking
Functional foods & nutraceuticals
Hyaluronic acid
Hydrogels
Lung diseases
Ophthalmology
Pharmaceuticals
Polymers
Skin
Surgery
Viscoelasticity
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Summary:Hyaluronic acid (HA), an excellent biomaterial with unique bio properties, is currently one of the most interesting polymers for many biomedical and cosmetic applications. However, several of its potential benefits are limited as it is rapidly degraded by hyaluronidase enzymes. To improve the half-life and consequently increase performance, native HA has been modified through cross-linking reactions with a natural and biocompatible amino acid, Ornithine, to overcome the potential toxicity commonly associated with traditional linkers. 2-chloro-dimethoxy-1,3,5-triazine/4-methylmorpholine (CDMT/NMM) was used as an activating agent. The new product (HA–Orn) was extensively characterized to confirm the chemical modification, and rheological analysis showed a gel-like profile. In vitro degradation experiments showed an improved resistance profile against enzymatic digestions. Furthermore, in vitro cytotoxicity studies were performed on lung cell lines (Calu-3 and H441), which showed no cytotoxicity.
ISSN:1999-4923
1999-4923
DOI:10.3390/pharmaceutics13101672