Artemether ameliorates kidney injury by restoring redox imbalance and improving mitochondrial function in Adriamycin nephropathy in mice

• Published in: Scientific reports 2021-01, Vol.11 (1), p.1266-1266, Article 1266
• Main Authors: Han, Pengxun, Cai, Yuchun, Wang, Yao, Weng, Wenci, Chen, Yinghui, Wang, Menghua, Zhan, Hongyue, Yu, Xuewen, Wang, Taifen, Shao, Mumin, Sun, Huili
• Format: Article
• Language: English
• Subjects: 692/308; 692/4022; Artemether; Biological effects; Copy number; Energy demand; Gene expression; Humanities and Social Sciences; Kidney diseases; Kidneys; Mitochondria; Mitochondrial DNA; Molecular modelling; multidisciplinary; Nephropathy; Phenotypes; Science; Science (multidisciplinary); Signal transduction; Transforming growth factor-b1
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-80298-x

The kidney is a high-energy demand organ rich in mitochondria especially renal tubular cells. Emerging evidence suggests that mitochondrial dysfunction, redox imbalance and kidney injury are interconnected. Artemether has biological effects by targeting mitochondria and exhibits potential therapeutic value for kidney disease. However, the underlying molecular mechanisms have not been fully elucidated. This study was performed to determine the effects of artemether on Adriamycin-induced nephropathy and the potential mechanisms were also investigated. In vivo, an Adriamycin nephropathy mouse model was established, and mice were treated with or without artemether for 2 weeks. In vitro, NRK-52E cells were stimulated with TGF-β1 and treated with or without artemether for 24 h. Then renal damage and cell changes were evaluated. The results demonstrated that artemether reduced urinary protein excretion, recovered podocyte alterations, attenuated pathological changes and alleviated renal tubular injury. Artemether also downregulated TGF-β1 mRNA expression levels, inhibited tubular proliferation, restored tubular cell phenotypes and suppressed proliferation-related signalling pathways. In addition, artemether restored renal redox imbalance, increased mtDNA copy number and improved mitochondrial function. In summary, we provided initial evidence that artemether ameliorates kidney injury by restoring redox imbalance and improving mitochondrial function in Adriamycin nephropathy in mice. Artemether may be a promising agent for the treatment kidney disease.