A long-lasting porcine model of ARDS caused by pneumonia and ventilator-induced lung injury

Animal models of acute respiratory distress syndrome (ARDS) do not completely resemble human ARDS, struggling translational research. We aimed to characterize a porcine model of ARDS induced by pneumonia-the most common risk factor in humans-and analyze the additional effect of ventilator-induced lu...

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Published in:Critical care (London, England) England), 2023-06, Vol.27 (1), p.239-239, Article 239
Main Authors: Barbeta, Enric, Arrieta, Marta, Motos, Ana, Bobi, Joaquim, Yang, Hua, Yang, Minlan, Tanzella, Giacomo, Di Ginnatale, Pierluigi, Nogas, Stefano, Vargas, Carmen Rosa, Cabrera, Roberto, Battaglini, Denise, Meli, Andrea, Kiarostami, Kasra, Vázquez, Nil, Fernández-Barat, Laia, Rigol, Montserrat, Mellado-Artigas, Ricard, Frigola, Gerard, Camprubí-Rimblas, Marta, Ferrer, Pau, Martinez, Daniel, Artigas, Antonio, Ferrando, Carlos, Ferrer, Miquel, Torres, Antoni
Format: Article
Language:English
Subjects:
Acute respiratory distress syndrome
Animals
ARDS
Bronchoscopy
Care and treatment
Complications and side effects
Critical care
Development and progression
Double hit
Drug resistance in microorganisms
Experiments
Hemodynamics
Injurious mechanical ventilation
Mechanics
Patient outcomes
Pneumonia
Porcine model
Prevention
Respiratory system
Ventilation
Ventilator-associated pneumonia
Ventilator-induced lung injury
Ventilators
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cited_by cdi_FETCH-LOGICAL-c564t-41437932076928d38061955890a4bb97f0c0cf95c9240ff6bdd99f6d34fb92ea3
cites cdi_FETCH-LOGICAL-c564t-41437932076928d38061955890a4bb97f0c0cf95c9240ff6bdd99f6d34fb92ea3
container_end_page 239
container_issue 1
container_start_page 239
container_title Critical care (London, England)
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creator Barbeta, Enric
Arrieta, Marta
Motos, Ana
Bobi, Joaquim
Yang, Hua
Yang, Minlan
Tanzella, Giacomo
Di Ginnatale, Pierluigi
Nogas, Stefano
Vargas, Carmen Rosa
Cabrera, Roberto
Battaglini, Denise
Meli, Andrea
Kiarostami, Kasra
Vázquez, Nil
Fernández-Barat, Laia
Rigol, Montserrat
Mellado-Artigas, Ricard
Frigola, Gerard
Camprubí-Rimblas, Marta
Ferrer, Pau
Martinez, Daniel
Artigas, Antonio
Ferrando, Carlos
Ferrer, Miquel
Torres, Antoni
description Animal models of acute respiratory distress syndrome (ARDS) do not completely resemble human ARDS, struggling translational research. We aimed to characterize a porcine model of ARDS induced by pneumonia-the most common risk factor in humans-and analyze the additional effect of ventilator-induced lung injury (VILI). Bronchoscopy-guided instillation of a multidrug-resistant Pseudomonas aeruginosa strain was performed in ten healthy pigs. In six animals (pneumonia-with-VILI group), pulmonary damage was further increased by VILI applied 3 h before instillation and until ARDS was diagnosed by PaO /FiO  
doi_str_mv 10.1186/s13054-023-04512-8
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We aimed to characterize a porcine model of ARDS induced by pneumonia-the most common risk factor in humans-and analyze the additional effect of ventilator-induced lung injury (VILI). Bronchoscopy-guided instillation of a multidrug-resistant Pseudomonas aeruginosa strain was performed in ten healthy pigs. In six animals (pneumonia-with-VILI group), pulmonary damage was further increased by VILI applied 3 h before instillation and until ARDS was diagnosed by PaO /FiO  &lt; 150 mmHg. Four animals (pneumonia-without-VILI group) were protectively ventilated 3 h before inoculum and thereafter. Gas exchange, respiratory mechanics, hemodynamics, microbiological studies and inflammatory markers were analyzed during the 96-h experiment. During necropsy, lobar samples were also analyzed. All animals from pneumonia-with-VILI group reached Berlin criteria for ARDS diagnosis until the end of experiment. The mean duration under ARDS diagnosis was 46.8 ± 7.7 h; the lowest PaO /FiO was 83 ± 5.45 mmHg. The group of pigs that were not subjected to VILI did not meet ARDS criteria, even when presenting with bilateral pneumonia. Animals developing ARDS presented hemodynamic instability as well as severe hypercapnia despite high-minute ventilation. Unlike the pneumonia-without-VILI group, the ARDS animals presented lower static compliance (p = 0.011) and increased pulmonary permeability (p = 0.013). The highest burden of P. aeruginosa was found at pneumonia diagnosis in all animals, as well as a high inflammatory response shown by a release of interleukin (IL)-6 and IL-8. At histological examination, only animals comprising the pneumonia-with-VILI group presented signs consistent with diffuse alveolar damage. 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We aimed to characterize a porcine model of ARDS induced by pneumonia-the most common risk factor in humans-and analyze the additional effect of ventilator-induced lung injury (VILI). Bronchoscopy-guided instillation of a multidrug-resistant Pseudomonas aeruginosa strain was performed in ten healthy pigs. In six animals (pneumonia-with-VILI group), pulmonary damage was further increased by VILI applied 3 h before instillation and until ARDS was diagnosed by PaO /FiO  &lt; 150 mmHg. Four animals (pneumonia-without-VILI group) were protectively ventilated 3 h before inoculum and thereafter. Gas exchange, respiratory mechanics, hemodynamics, microbiological studies and inflammatory markers were analyzed during the 96-h experiment. During necropsy, lobar samples were also analyzed. All animals from pneumonia-with-VILI group reached Berlin criteria for ARDS diagnosis until the end of experiment. The mean duration under ARDS diagnosis was 46.8 ± 7.7 h; the lowest PaO /FiO was 83 ± 5.45 mmHg. The group of pigs that were not subjected to VILI did not meet ARDS criteria, even when presenting with bilateral pneumonia. Animals developing ARDS presented hemodynamic instability as well as severe hypercapnia despite high-minute ventilation. Unlike the pneumonia-without-VILI group, the ARDS animals presented lower static compliance (p = 0.011) and increased pulmonary permeability (p = 0.013). The highest burden of P. aeruginosa was found at pneumonia diagnosis in all animals, as well as a high inflammatory response shown by a release of interleukin (IL)-6 and IL-8. At histological examination, only animals comprising the pneumonia-with-VILI group presented signs consistent with diffuse alveolar damage. In conclusion, we established an accurate pulmonary sepsis-induced ARDS model.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>37328874</pmid><doi>10.1186/s13054-023-04512-8</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record>
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ispartof Critical care (London, England), 2023-06, Vol.27 (1), p.239-239, Article 239
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source Open Access: PubMed Central; Publicly Available Content (ProQuest)
subjects Acute respiratory distress syndrome
Animals
ARDS
Bronchoscopy
Care and treatment
Complications and side effects
Critical care
Development and progression
Double hit
Drug resistance in microorganisms
Experiments
Hemodynamics
Injurious mechanical ventilation
Mechanics
Patient outcomes
Pneumonia
Porcine model
Prevention
Respiratory system
Ventilation
Ventilator-associated pneumonia
Ventilator-induced lung injury
Ventilators
title A long-lasting porcine model of ARDS caused by pneumonia and ventilator-induced lung injury
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T15%3A25%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20long-lasting%20porcine%20model%20of%20ARDS%20caused%20by%20pneumonia%20and%20ventilator-induced%20lung%20injury&rft.jtitle=Critical%20care%20(London,%20England)&rft.au=Barbeta,%20Enric&rft.date=2023-06-16&rft.volume=27&rft.issue=1&rft.spage=239&rft.epage=239&rft.pages=239-239&rft.artnum=239&rft.issn=1364-8535&rft.eissn=1466-609X&rft_id=info:doi/10.1186/s13054-023-04512-8&rft_dat=%3Cgale_doaj_%3EA753260137%3C/gale_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c564t-41437932076928d38061955890a4bb97f0c0cf95c9240ff6bdd99f6d34fb92ea3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2827103376&rft_id=info:pmid/37328874&rft_galeid=A753260137&rfr_iscdi=true