Molecular Surveillance for Imported Antimicrobial Resistant Plasmodium falciparum, Ontario, Canada

Single-nucleotide polymorphisms at several loci have been correlated with Plasmodium falciparum drug resistance. We examined the prevalence of resistance markers in P. falciparum from imported malaria cases in Canada during 3 time periods, 2008-2009, 2013-2014, and 2017-2018. We evaluated single-nuc...

Full description

Saved in:
Bibliographic Details
Published in:Emerging infectious diseases 2022-04, Vol.28 (4), p.812-819
Main Authors: Kariyawasam, Ruwandi, Lau, Rachel, Shao, Eric, Tan, Katherine, Showler, Adrienne, Ralevski, Filip, Patel, Samir N, Boggild, Andrea K
Format: Article
Language:English
Subjects:
Anti-Infective Agents
Antimalarials - pharmacology
Antimalarials - therapeutic use
Antimicrobial agents
antimicrobial resistance
Biobanks
Design
Dihydrofolate reductase
Drug resistance
Drug resistance in microorganisms
Erythrocytes
Genes
Genetic aspects
Genotypes
Health surveillance
Identification and classification
Infections
Malaria
Molecular Surveillance for Imported Antimicrobial Resistant
Mutation
Ontario
Ontario, Canada
parasites
Parasitological research
Plasmodium
Plasmodium falciparum
Plasmodium falciparum - genetics
Protozoan Proteins - genetics
Risk factors
Single nucleotide polymorphisms
Software
Statistics
Vector-borne diseases
vector-borne infections
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Single-nucleotide polymorphisms at several loci have been correlated with Plasmodium falciparum drug resistance. We examined the prevalence of resistance markers in P. falciparum from imported malaria cases in Canada during 3 time periods, 2008-2009, 2013-2014, and 2017-2018. We evaluated single-nucleotide polymorphisms at atpase6 (pfATPase6), pfcrt (chloroquine resistance transporter), cytb (cytochrome b), dhfr (dihydrofolate reductase), dhps (dihydropteroate synthetase), mdr1 (multidrug resistance protein) and mdr1 copy number, and kelch13 (kelch protein gene on chromosome 13). Over time, we observed increasing mutant genotypes for dhfr S108N and dhps A613T and decreasing mutant genotypes for mdr1 N86Y, D1246Y, pfcrt K76T, and pfcrt 74-75; we identified no kelch13 mutations. We observed fewer mutations indicative of chloroquine resistance over time, which may reflect reduced chloroquine pressure in specimens from travelers to Africa. Mutations conferring proguanil resistance increased over time. Minor genotypes confirm the heterogeneous nature of infection and may affect treatment success.
ISSN:1080-6040
1080-6059
1080-6059
DOI:10.3201/eid2804.210533