One-Step Partially Purified Lipases (ScLipA and ScLipB) from Schizophyllum commune UTARA1 Obtained via Solid State Fermentation and Their Applications

• Published in: Molecules (Basel, Switzerland) Switzerland), 2017-12, Vol.22 (12), p.2106
• Main Authors: Kam, Yew Chee, Woo, Kwan Kit, Ong, Lisa Gaik Ai
• Format: Article
• Language: English
• Subjects: Affinity; Ammonium sulfate; Bagasse; Chains; Coconut oil; Cooking; Cooking oils; Crude oil; Design optimization; Esterification; Fermentation; Fish oils; Fish Oils - chemistry; Glycerol; Glycerol - chemistry; Hydrolysis; Industrial applications; Kinetics; Lipase; Lipase - chemistry; Lipase - isolation & purification; Lipase - metabolism; Oils & fats; Oleic Acids - chemistry; Olein; Plant Oils - chemistry; Purification; Schizophyllum - enzymology; Solid state; Solid state fermentation; Studies; Sugarcane; sugarcane bagasse; Triacetin; Triacetin - chemistry; Triglycerides; Triglycerides - chemistry; used cooking oil
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules22122106

Lipases with unique characteristics are of value in industrial applications, especially those targeting cost-effectiveness and less downstream processes. The aims of this research were to: (i) optimize the fermentation parameters via solid state fermentation (SSF); and (ii) study the performance in hydrolysis and esterification processes of the one-step partially purified UTARA1 lipases. Lipase was produced by cultivating UTARA1 on sugarcane bagasse (SB) with used cooking oil (UCO) via SSF and its production was optimized using Design-Expert 7.0.0. Fractions 30% ( LipA) and 70% ( LipB) which contained high lipase activity were obtained by stepwise (NH₄)₂SO₄ precipitation. Crude fish oil, coconut oil and butter were used to investigate the lipase hydrolysis capabilities by a free glycerol assay. Results showed that LipA has affinities for long, medium and short chain triglycerides, as all the oils investigated were degraded, whereas LipB has affinities for long chain triglycerides as it only degrades crude fish oil. During esterification, LipA was able to synthesize trilaurin and triacetin. Conversely, LipB was specific towards the formation of 2-mono-olein and triacetin. From the results obtained, it was determined that LipA and LipB are -2 regioselective lipases. Hence, the one-step partial purification strategy proved to be feasible for partial purification of UTARA1 lipases that has potential use in industrial applications.