Perspective of a new diagnostic for human trichomonosis

Several diagnostic techniques have been employed for the detection of Trichomonas vaginalis. Microtubules constitute the cytoskeleton in eukaryotic cells and are sensitive to antimitotic drugs, such as Taxol (paclitaxel). We used FLUTAX a fluorescent taxoid - to analyze the microtubule distribution...

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Bibliographic Details
Published in:Memórias do Instituto Oswaldo Cruz 2003-03, Vol.98 (2), p.273-276
Main Authors: Lecke, Sheila B, Tasca, Tiana, Souto, André A, De Carli, Geraldo A
Format: Article
Language:English
Subjects:
Animals
Centrosome - ultrastructure
Cytoskeleton - ultrastructure
diagnostic techniques
Female
Flagella - ultrastructure
Fluorescent Dyes
FLUTAX
Humans
Microscopy, Fluorescence
Microtubules - ultrastructure
Paclitaxel - analogs & derivatives
PARASITOLOGY
Taxoids
Trichomonas vaginalis
Trichomonas vaginalis - ultrastructure
Trichomonas Vaginitis - diagnosis
TROPICAL MEDICINE
Vaginal Discharge - parasitology
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Summary:Several diagnostic techniques have been employed for the detection of Trichomonas vaginalis. Microtubules constitute the cytoskeleton in eukaryotic cells and are sensitive to antimitotic drugs, such as Taxol (paclitaxel). We used FLUTAX a fluorescent taxoid - to analyze the microtubule distribution in living trophozoites of T. vaginalis in urine and in vaginal discharge. A high intensity of fluorescence was observed in living T. vaginalis, epithelial cells and leukocytes present in urine and vaginal discharge. Our preliminary results show the perspective of a new diagnostic technique for trichomonosis and will contribute to the understanding of the cytoskeleton of T. vaginalis.
ISSN:0074-0276
1678-8060
0074-0276
1678-8060
DOI:10.1590/S0074-02762003000200018