Efficient in vitro direct shoot organogenesis from seedling derived split node explants of maize (Zea mays L.)

Maize is one of the important cereal crops around the world. An efficient and reproducible regeneration protocol via direct organogenesis has been established using split nodes as ex-plants derived from 7 to 10day old in vitro grown seedlings. Surface sterilized maize seeds were germinated on MS med...

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Bibliographic Details
Published in:Journal of Genetic Engineering and Biotechnology 2016-06, Vol.14 (1), p.49-53
Main Authors: Mushke, Ramesh, Yarra, Rajesh, Bulle, Mallesham
Format: Article
Language:English
Subjects:
abiotic stress
acclimation
benzyladenine
callus
corn
Explants
genetic engineering
grain crops
greenhouse production
I : Plant Biotechnology
indole butyric acid
kinetin
Maize
nutritive value
organogenesis
plantlets
roots
seedlings
seeds
Shoot organogenesis
shoots
Split node
Zea mays
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Summary:Maize is one of the important cereal crops around the world. An efficient and reproducible regeneration protocol via direct organogenesis has been established using split nodes as ex-plants derived from 7 to 10day old in vitro grown seedlings. Surface sterilized maize seeds were germinated on MS medium lacking plant growth regulators. Nodal sections of 7–10day old seedlings were isolated, split longitudinally into two halves and cultured on regeneration medium containing different concentrations of 6-benzyladenine (2.20, 4.40, 6.60, 8.80, 11.0 and 13.2μM) or kinetin (2.32, 4.65, 6.97, 9.29, 11.6 and 13.9μM). Inclusion of 8.80μM BA into MS supplemented medium triggered a high frequency of regeneration response from split node explants with a maximum number of shoots (12.0±1.15) and the highest shoot length (3.0±0.73) was obtained directly (without an intervening callus phase) within 4weeks of culture. Further shoot elongation was achieved on medium containing 4.40μM BA. The elongated micro shoots were rooted on MS medium fortified with 1.97μM indole-3-butyric acid. The regenerated plantlets with roots were successfully hardened on earthen pots after proper acclimatization under greenhouse conditions. This new efficient regeneration method provides a solid foundation for genetic manipulation of maize for biotic and abiotic stresses and to enhance the nutritional values.
ISSN:1687-157X
2090-5920
DOI:10.1016/j.jgeb.2016.03.001