Detection and imaging of Hg(II) in vivo using glutathione-functionalized gold nanoparticles

The optical and biological properties of functionalized gold nanoparticles (GNPs) have been widely used in sensing applications. GNPs have a strong binding ability to thiol groups. Furthermore, thiols are used to bind functional molecules, which can then be used, for example, to detect metal ions in...

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Bibliographic Details
Published in:Beilstein journal of nanotechnology 2022-06, Vol.13 (1), p.549-559
Main Authors: Li, Gufeng, Li, Shaoqing, Wang, Rui, Yang, Min, Zhang, Lizhu, Zhang, Yanli, Yang, Wenrong, Wang, Hongbin
Format: Article
Language:English
Subjects:
Biocompatibility
Biological properties
cell imaging
Cytotoxicity
Fluorescence
fluorescence probes
Full Research Paper
Glutathione
Gold
gold nanoparticles
Mercury (metal)
mercury ions
Nanoparticles
Nanoscience
Nanotechnology
Optical properties
Rhodamine 6G
rhodamine 6g derivatives
Thiols
Toxicity
Vibration
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Summary:The optical and biological properties of functionalized gold nanoparticles (GNPs) have been widely used in sensing applications. GNPs have a strong binding ability to thiol groups. Furthermore, thiols are used to bind functional molecules, which can then be used, for example, to detect metal ions in solution. Herein, we describe 13 nm GNPs functionalized by glutathione (GSH) and conjugated with a rhodamine 6G derivative (Rh6G2), which can be used to detect Hg(II) in cells. The detection of Hg 2+ ions is based on an ion-catalyzed hydrolysis of the spirolactam ring of Rh6G2, leading to a significant change in the fluorescence of GNPs-GSH-Rh6G2 from an “OFF” to an “ON” state. This strategy is an effective tool to detect Hg 2+ ions. In cytotoxicity experiments, GNPs-GSH-Rh6G2 could penetrate living cells and detect mercury ions through the fluorescent “ON” form.
ISSN:2190-4286
2190-4286
DOI:10.3762/bjnano.13.46