Induction of Pluripotency in Adult Equine Fibroblasts without c-MYC

Despite tremendous efforts on isolation of pluripotent equine embryonic stem (ES) cells, to date there are few reports about successful isolation of ESCs and no report of in vivo differentiation of this important companion species. We report the induction of pluripotency in adult equine fibroblasts...

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Published in:Stem Cells International 2012-01, Vol.2012 (2012), p.480-488
Main Authors: Williamson, Mark, Lim, Susan, Pashaiasl, Maryam, Sumer, Huseyin, Khodadadi, Khodadad, Verma, Paul J.
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container_end_page 488
container_issue 2012
container_start_page 480
container_title Stem Cells International
container_volume 2012
creator Williamson, Mark
Lim, Susan
Pashaiasl, Maryam
Sumer, Huseyin
Khodadadi, Khodadad
Verma, Paul J.
description Despite tremendous efforts on isolation of pluripotent equine embryonic stem (ES) cells, to date there are few reports about successful isolation of ESCs and no report of in vivo differentiation of this important companion species. We report the induction of pluripotency in adult equine fibroblasts via retroviral transduction with three transcription factors using OCT4, SOX2, and KLF4 in the absence of c-MYC. The cell lines were maintained beyond 27 passages (more than 11 months) and characterized. The equine iPS (EiPS) cells stained positive for alkaline phosphatase by histochemical staining and expressed OCT4, NANOG, SSEA1, and SSEA4. Gene expression analysis of the cells showed the expression of OCT4, SOX2 NANOG, and STAT3. The cell lines retained a euploid chromosome count of 64 after long-term culture cryopreservation. The EiPS demonstrated differentiation capacity for the three embryonic germ layers both in vitro by embryoid bodies (EBs) formation and in vivo by teratoma formation. In conclusion, we report the derivation of iPS cells from equine adult fibroblasts and long-term maintenance using either of the three reprogramming factors.
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title Induction of Pluripotency in Adult Equine Fibroblasts without c-MYC
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