Differential Mobilization of the Phospholipid and Triacylglycerol Pools of Arachidonic Acid in Murine Macrophages

Innate immune cells such as monocytes and macrophages contain high levels of arachidonic acid (AA), part of which can be mobilized during cellular activation for the formation of a vast array of bioactive oxygenated metabolites. Monocytes and macrophages present in inflammatory foci typically incorp...

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Bibliographic Details
Published in:Biomolecules (Basel, Switzerland) Switzerland), 2022-12, Vol.12 (12), p.1851
Main Authors: Bermúdez, Miguel A, Rubio, Julio M, Balboa, María A, Balsinde, Jesús
Format: Article
Language:English
Subjects:
Animals
Arachidonic acid
Arachidonic Acid - metabolism
Cell activation
Cell culture
Chromatography
Fatty acids
Inflammation
Lipids
Macrophages
Macrophages - metabolism
membrane phospholipid
Mice
Monocytes
monocytes/macrophages
phospholipase A2
Phospholipids
Phospholipids - metabolism
Physiological aspects
triacylglycerol
Triglycerides
Triglycerides - metabolism
Zymosan - pharmacology
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Summary:Innate immune cells such as monocytes and macrophages contain high levels of arachidonic acid (AA), part of which can be mobilized during cellular activation for the formation of a vast array of bioactive oxygenated metabolites. Monocytes and macrophages present in inflammatory foci typically incorporate large amounts of AA, not only in membrane phospholipids, but also in neutral lipids such as triacylglycerol. Thus, it was of interest to investigate the metabolic fate of these two AA pools in macrophages. Utilizing a variety of radiolabeling techniques to distinguish the phospholipid and triacylglycerol pools, we show in this paper that during an acute stimulation of the macrophages with yeast-derived zymosan, the membrane phospholipid AA pool acts as the major, if not the only, source of releasable AA. On the contrary, the AA pool in triacylglycerol appears to be used at a later stage, when the zymosan-stimulated response has declined, as a source to replenish the phospholipid pools that were consumed during the activation process. Thus, phospholipids and triacylglycerol play different in roles AA metabolism and dynamics during macrophage activation.
ISSN:2218-273X
2218-273X
DOI:10.3390/biom12121851