Competitive HRP-Linked Colorimetric Aptasensor for the Detection of Fumonisin B1 in Food based on Dual Biotin-Streptavidin Interaction

Fumonisin B1 (FB1) is the most prevalent and toxic form among fumonisin homologues which are produced by fusarium species and it contaminates various types of food products, posing serious health hazards for humans and animals. In this work, a colorimetric assay for the detection of FB1 has been dev...

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Bibliographic Details
Published in:Biosensors (Basel) 2020-03, Vol.10 (4), p.31
Main Authors: Tao, Zui, Zhou, You, Li, Xiang, Wang, Zhouping
Format: Article
Language:English
Subjects:
Animals
aptamer
Aptamers
Assaying
Beer
Binding
Biotin
Biotin - chemistry
Chromatography
Colorimetry
Colorimetry - methods
Food
Food Analysis - methods
Food production
food safety
Fumonisin B1
Fumonisins - chemistry
Health hazards
Homology
Horseradish peroxidase
Humans
Linearity
mycotoxin
Peroxidase
Sorghum
Streptavidin
Streptavidin - chemistry
Visual observation
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Summary:Fumonisin B1 (FB1) is the most prevalent and toxic form among fumonisin homologues which are produced by fusarium species and it contaminates various types of food products, posing serious health hazards for humans and animals. In this work, a colorimetric assay for the detection of FB1 has been developed based on competitive horseradish peroxidase (HRP)-linked aptamer and dual biotin-streptavidin interaction. In short, a biotinylated aptamer of FB1 was immobilized on the microplate by biotin-streptavidin binding; the complementary strand (csDNA) of the aptamer was ligated with HRP by biotin-streptavidin binding again to form a csDNA-HRP sensing probe, competing with FB1 to bind to the aptamer. The color change can be observed after the addition of chromogenic and stop solution, thereby realizing the visual detection of FB1. Under optimal conditions, good linearity was observed within the concentration range of 0.5 to 300 ng/mL, with a detection of limit of 0.3 ng/mL. This assay is further validated by spike recovery tests towards beer and corn samples, it provides a simple, sensitive and reliable method for the screening of FB1 in food samples and may be potentially used as an alternative to conventional assays.
ISSN:2079-6374
2079-6374
DOI:10.3390/bios10040031