Effect of Sainfoin (Onobrychis viciifolia) Pellets on Rumen Microbiome and Histopathology in Lambs Exposed to Gastrointestinal Nematodes

Our study analyzed the ruminal fermentation and microbiome, hematological profile, and abomasal histopathology of lambs experimentally infected with a gastrointestinal nematode (GIN) and fed sainfoin pellets (SFPs; 600 g DM/d/animal) for 14 d. Twenty-four lambs infected with Haemonchus contortus wer...

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Published in:Agriculture (Basel) 2022-02, Vol.12 (2), p.301
Main Authors: Petrič, Daniel, Komáromyová, Michaela, Batťányi, Dominika, Kozłowska, Martyna, Filipiak, Weronika, Łukomska, Anna, Ślusarczyk, Sylwester, Szumacher-Strabel, Malgorzata, Cieślak, Adam, Várady, Marián, Kišidayová, Svetlana, Váradyová, Zora
Format: Article
Language:English
Subjects:
Ammonia
Animals
Archaea
bacteria
Emissions
Erythrocytes
Experiments
Fermentation
Flavonoids
Gases
hematological profiles
Histopathology
In vivo methods and tests
Intestinal parasites
Laboratory animals
Methane
methane concentration
Methanogenic bacteria
Microbiomes
Nematodes
Nitrogen
Nutrition
Parasites
Pellets
plant bioactive components
Relative abundance
ruminal fermentation
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Summary:Our study analyzed the ruminal fermentation and microbiome, hematological profile, and abomasal histopathology of lambs experimentally infected with a gastrointestinal nematode (GIN) and fed sainfoin pellets (SFPs; 600 g DM/d/animal) for 14 d. Twenty-four lambs infected with Haemonchus contortus were divided into two separated groups: animals fed meadow hay (control) and animals fed SFPs. The ruminal contents, fermentation parameters, and microbiome in vitro and in vivo were determined using molecular and microscopic techniques. Ruminal contents in the SFP group indicated smaller populations of Archaea (p < 0.001), Methanomicrobiales (p = 0.009), and lower methane concentrations in vitro (p = 0.046) and in vivo (p = 0.030) than the control group. The relative abundance of Butyrivibrio fibrisolvens quantified by real-time PCR was higher in the lambs with the SFP diet (p = 0.05). Haemonchosis affected the number of red blood cells of the lambs (p < 0.001). The lambs in the SFP group had a higher percentage of damaged abomasa glands than did the control group (p = 0.004). The consumption of SFPs by GIN-infected lambs may affect ruminal methanogens and subsequently decrease methane emission without undesirable changes in the ruminal microbiome or the health of the animals.
ISSN:2077-0472
2077-0472
DOI:10.3390/agriculture12020301