Ether-Oxygen Containing Electrospun Microfibrous and Sub-Microfibrous Scaffolds Based on Poly(butylene 1,4-cyclohexanedicarboxylate) for Skeletal Muscle Tissue Engineering

We report the study of novel biodegradable electrospun scaffolds from poly(butylene 1,4-cyclohexandicarboxylate- -triethylene cyclohexanedicarboxylate) (P(BCE- -TECE)) as support for in vitro and in vivo muscle tissue regeneration. We demonstrate that chemical composition, i.e., the amount of TECE c...

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Bibliographic Details
Published in:International journal of molecular sciences 2018-10, Vol.19 (10), p.3212
Main Authors: Bloise, Nora, Berardi, Emanuele, Gualandi, Chiara, Zaghi, Elisa, Gigli, Matteo, Duelen, Robin, Ceccarelli, Gabriele, Cortesi, Emanuela Elsa, Costamagna, Domiziana, Bruni, Giovanna, Lotti, Nadia, Focarete, Maria Letizia, Visai, Livia, Sampaolesi, Maurilio
Format: Article
Language:English
Subjects:
Animals
biodegradable polyesters
Cell Differentiation
Cell Line
Cell Proliferation
Cell Shape
Cyclohexanes - chemistry
electrospinning
Implants, Experimental
Inflammation - pathology
Ki-67 Antigen - metabolism
Male
Mice, Inbred C57BL
microfibres and sub-microfibres
muscle tissue engineering
Muscle, Skeletal - physiology
myogenesis
Neovascularization, Physiologic
Oxygen - chemistry
Polyenes - chemistry
Polyethylene Glycols - chemistry
Tissue Engineering - methods
Tissue Scaffolds - chemistry
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Summary:We report the study of novel biodegradable electrospun scaffolds from poly(butylene 1,4-cyclohexandicarboxylate- -triethylene cyclohexanedicarboxylate) (P(BCE- -TECE)) as support for in vitro and in vivo muscle tissue regeneration. We demonstrate that chemical composition, i.e., the amount of TECE co-units (constituted of polyethylene glycol-like moieties), and fibre morphology, i.e., aligned microfibrous or sub-microfibrous scaffolds, are crucial in determining the material biocompatibility. Indeed, the presence of ether linkages influences surface wettability, mechanical properties, hydrolytic degradation rate, and density of cell anchoring points of the studied materials. On the other hand, electrospun scaffolds improve cell adhesion, proliferation, and differentiation by favouring cell alignment along fibre direction (fibre morphology), also allowing for better cell infiltration and oxygen and nutrient diffusion (fibre size). Overall, C2C12 myogenic cells highly differentiated into mature myotubes when cultured on microfibres realised with the copolymer richest in TECE co-units (micro-P73 mat). Lastly, when transplanted in the tibialis anterior muscles of healthy, injured, or dystrophic mice, micro-P73 mat appeared highly vascularised, colonised by murine cells and perfectly integrated with host muscles, thus confirming the suitability of P(BCE- -TECE) scaffolds as substrates for skeletal muscle tissue engineering.
ISSN:1422-0067
1422-0067
DOI:10.3390/ijms19103212