Preparation and in vivo evaluation of glyco-gold nanoparticles carrying synthetic mycobacterial hexaarabinofuranoside

A number of bacterial glycans are specific markers for the detection and the serological identification of microorganisms and are also widely used as antigenic components of vaccines. The use of gold nanoparticles as carriers for glyco-epitopes is becoming an important alternative to the traditional...

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Bibliographic Details
Published in:Beilstein journal of nanotechnology 2020, Vol.11 (1), p.480-493
Main Authors: Burygin, Gennady L, Abronina, Polina I, Podvalnyy, Nikita M, Staroverov, Sergey A, Kononov, Leonid O, Dykman, Lev A
Format: Article
Language:English
Subjects:
Antibodies
Antigens
Carbohydrates
Conjugation
conjugation of glycosides
Dielectric properties
E coli
Full Research Paper
Gold
gold nanoparticles
Immunization
Immunoglobulins
In vivo methods and tests
Ligands
lipoarabinomannan
Microorganisms
mycobacterium
Nanoparticles
Nanoscience
Nanotechnology
Polysaccharides
Rabbits
spacer aglycon
Transmission electron microscopy
Tuberculosis
Vaccines
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Summary:A number of bacterial glycans are specific markers for the detection and the serological identification of microorganisms and are also widely used as antigenic components of vaccines. The use of gold nanoparticles as carriers for glyco-epitopes is becoming an important alternative to the traditional conjugation with proteins and synthetic polymers. In this study, we aimed to prepare and evaluate in vivo glyco-gold nanoparticles (glyco-GNPs) bearing the terminal-branched hexaarabinofuranoside fragment (Ara ) of arabinan domains of lipoarabinomannan and arabinogalactan, which are principal polysaccharides of the cell wall of , the causative agent of tuberculosis. In particular, we were interested whether the antibodies generated against Ara -GNPs would recognize the natural saccharides on the cell surface of different mycobacterial strains. Two synthetic Ara glycosides with amino-functionalized spacer aglycons differing in length and hydrophilicity were directly conjugated with spherical gold nanoparticles ( = 15 nm) to give two sets of glyco-GNPs, which were used for the immunization of rabbits. Dot assays revealed cross-reactions between the two obtained antisera with the hexaarabinofuranoside and the 2-aminoethyl aglycon used for the preparation of glyco-GNPs. Both antisera contained high titers of antibodies specific for as shown by enzyme-linked immunosorbent assay using and cells as antigens while there was only a weak response to cells and no interaction with cells. The results obtained suggest that glyco-GNPs are promising agents for the generation of anti-mycobacterial antibodies.
ISSN:2190-4286
2190-4286
DOI:10.3762/bjnano.11.39