The key role of CYC2 during meiosis in Tetrahymena thermophila

Meiotic recombination is carried out through a special- ized pathway for the formation and repair of DNA dou- ble-strand breaks （DSBs） made by the Spo11 protein. The present study shed light on the functional role of cyclin, CYC2, in Tetrahymena thermophila which has transcriptionally high expressio...

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Bibliographic Details
Published in:Protein & cell 2016-04, Vol.7 (4), p.236-249
Main Authors: Xu, Qianlan, Wang, Ruoyu, Ghanam, A. R., Yan, Guanxiong, Miao, Wei, Song, Xiaoyuan
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Cell Biology
Cell Cycle Checkpoints
cyclin
Cyclins - genetics
Cyclins - metabolism
Developmental Biology
DNA Breaks, Double-Stranded
DNA Mismatch Repair
DNA Repair
DNA修复
DNA双链断裂
Endodeoxyribonucleases - genetics
Endodeoxyribonucleases - metabolism
Homologous Recombination
Human Genetics
Life Sciences
Meiosis
Microscopy, Fluorescence
Phenotype
Protein Science
Protozoan Proteins - genetics
Protozoan Proteins - metabolism
Real-Time Polymerase Chain Reaction
Research Article
RNA-Seq
Sequence Analysis, RNA
Stem Cells
Tetrahymena thermophila
Tetrahymena thermophila - genetics
Tetrahymena thermophila - metabolism
Transcriptome
减数分裂
分裂过程
四膜虫
基因敲除
差异表达基因
细胞周期蛋白
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Summary:Meiotic recombination is carried out through a special- ized pathway for the formation and repair of DNA dou- ble-strand breaks （DSBs） made by the Spo11 protein. The present study shed light on the functional role of cyclin, CYC2, in Tetrahymena thermophila which has transcriptionally high expression level during meiosis process. Knocking out the CYC2 gene results in arrest of meiotic conjugation process at 2.5-3.5 h after conjugation initiation, before the meiosis division starts, and in company with the absence of DSBs. To investigate the underlying mechanism of this phenomenon, a complete transcriptome profile was performed between wild-type strain and CYC2 knock-out strain. Functional analysis of RNA-Seq results identifies related differentially expressed genes （DEGs） including SP011 and these DEGs are enriched in DNA repair/mismatch repair （MMR） terms in homologous recombination （HR）, which indicates that CYC2 could play a crucial role in meiosis by regulating SP011 and participating in HR.
ISSN:1674-800X
1674-8018
DOI:10.1007/s13238-016-0254-9