Evidence for the Involvement of the Chemosensory Protein AgosCSP5 in Resistance to Insecticides in the Cotton Aphid, Aphis gossypii

It has been speculated that insect chemosensory proteins (CSPs) may have additional roles beyond olfaction. In this study, the phylogenetic and genomic analyses of the CSPs of the cotton aphid, , revealed the presence of gene gain-and-loss among different aphid field populations. Differential expres...

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Bibliographic Details
Published in:Insects (Basel, Switzerland) Switzerland), 2021-04, Vol.12 (4), p.335
Main Authors: Li, Fen, Venthur, Herbert, Wang, Shang, Homem, Rafael A, Zhou, Jing-Jiang
Format: Article
Language:English
Subjects:
Aphis gossypii
Binding
Bioassays
Chemoreception
chemosensory protein
Cotton
CSP gene
CSP protein
Cypermethrin
Deltamethrin
Drosophila
Dynamic stability
Fluvalinate
Fruit flies
Gene expression
Genes
Genomes
Genomic analysis
Homology
Host location
Imidacloprid
In vivo methods and tests
insect pest management
Insecticide resistance
Insecticides
Insects
Ligands
Maximum likelihood method
Molecular docking
Olfaction
Omethoate
Pest control
Pesticide resistance
Pests
Phylogeny
Physiology
Populations
Proteins
Regeneration
Signal transduction
Tissue engineering
Transgenic animals
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Summary:It has been speculated that insect chemosensory proteins (CSPs) may have additional roles beyond olfaction. In this study, the phylogenetic and genomic analyses of the CSPs of the cotton aphid, , revealed the presence of gene gain-and-loss among different aphid field populations. Differential expressions of eight genes were demonstrated after treatments with insecticides of different modes of action. The expression of was significantly upregulated by the insecticide treatments in a dose-dependent manner. The flies overexpressing were significantly less susceptible to the insecticides, omethoate, imidacloprid and cypermethrin but not to deltamethrin and tau-fluvalinate, compared with control flies. The transgenic flies exhibited an LC resistance ratio of 2.6 to omethoate, compared with control flies. Likewise, the mortality of the transgenic flies to imidacloprid and cypermethrin was significantly lower than that of the control flies ( < 0.01). Homology modelling, molecular docking and dynamic simulation supported the interactions and revealed a higher stability of AgosCSP5/insecticide complexes than AgosCSP5/semiochemical complexes. Our study demonstrates for first time the in vivo evidence for the involvement of genes in insecticide resistance of crop insect pests and provides new insights of the newly discovered CSP-mediated insect resistance mechanism to insecticides.
ISSN:2075-4450
2075-4450
DOI:10.3390/insects12040335