Proteomic and Bioinformatic Tools to Identify Potential Hub Proteins in the Audiogenic Seizure-Prone Hamster GASH/Sal

The GASH/Sal (Genetic Audiogenic Seizure Hamster, Salamanca) is a model of audiogenic seizures with the epileptogenic focus localized in the inferior colliculus (IC). The sound-induced seizures exhibit a short latency (7-9 s), which implies innate protein disturbances in the IC as a basis for seizur...

Full description

Saved in:
Bibliographic Details
Published in:Diagnostics (Basel) 2023-03, Vol.13 (6), p.1048
Main Authors: García-Peral, Carlos, Ledesma, Martín M, Herrero-Turrión, M Javier, Gómez-Nieto, Ricardo, Castellano, Orlando, López, Dolores E
Format: Article
Language:English
Subjects:
Acoustics
Brain research
Computational biology
Convulsions & seizures
Epilepsy
epileptogenesis
epileptogenic nucleus
GASH/Sal hamster strain
Genetic aspects
genetic epilepsy models
Health aspects
Inferior colliculi
Ions
Laboratory animals
Metabolism
Peptides
Physiology
Proteins
proteomic profile
Proteomics
Seizures (Medicine)
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The GASH/Sal (Genetic Audiogenic Seizure Hamster, Salamanca) is a model of audiogenic seizures with the epileptogenic focus localized in the inferior colliculus (IC). The sound-induced seizures exhibit a short latency (7-9 s), which implies innate protein disturbances in the IC as a basis for seizure susceptibility and generation. Here, we aim to study the protein profile in the GASH/Sal IC in comparison to controls. Protein samples from the IC were processed for enzymatic digestion and then analyzed by mass spectrometry in Data-Independent Acquisition mode. After identifying the proteins using the UniProt database, we selected those with differential expression and performed ontological analyses, as well as gene-protein interaction studies using bioinformatics tools. We identified 5254 proteins; among them, 184 were differentially expressed proteins (DEPs), with 126 upregulated and 58 downregulated proteins, and 10 of the DEPs directly related to epilepsy. Moreover, 12 and 7 proteins were uniquely found in the GASH/Sal or the control. The results indicated a protein profile alteration in the epileptogenic nucleus that might underlie the inborn occurring audiogenic seizures in the GASH/Sal model. In summary, this study supports the use of bioinformatics methods in proteomics to delve into the relationship between molecular-level protein mechanisms and the pathobiology of rodent models of audiogenic seizures.
ISSN:2075-4418
2075-4418
DOI:10.3390/diagnostics13061048